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Updated: Jan 25, 2026

Dynamic Continuous Blood Extraction from Rat Heart via Noninvasive Microdialysis Technique
Published on: September 13, 2022
Determination of Dermal Pharmacokinetics by Microdialysis Sampling in Rats
Nivea M F Voelkner1,2,3, Alexander Voelkner1, Hartmut Derendorf1
1Department of Pharmaceutics, College of Pharmacy, University of Florida, Gainesville, Florida.
Abstract:
The evaluation of absorption and availability at the site of action of a drug candidate is an important element of drug discovery and development, as clinical response is a function of the bioavailability of the active agent and its continued presence at the site of action. Evaluation of dermal pharmacokinetics facilitates the selection of new compounds or chemical structures for advancement as possible clinical candidates. An advantage of microdialysis is that it allows the measurement of compound concentrations at the site of action without disturbing the tissue milieu, making it possible to determine the relationship between this important variable and plasma concentrations of the agent. Described in this unit are laboratory protocols for performing dermal microdialysis experiments in rat for the purpose of defining the pharmacokinetics parameters of test agents. © 2019 by John Wiley & Sons, Inc.
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