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Rapid and sensitive determination of bisphenol A using aptamer and split DNAzyme
Jing Xu1, Eun-Song Lee2, Myung Chan Gye3
1Department of Environmental Sciences, Hanyang University, Seoul, 04763, Republic of Korea.
Chemosphere
|April 27, 2019
Summary
A new nucleic acid-based method offers sensitive detection of bisphenol A (BPA), an endocrine disrupting chemical (EDC). This simple approach uses DNA aptamers and DNAzymes for rapid and selective BPA monitoring in environmental samples.
Area of Science:
- Environmental Chemistry
- Biotechnology
- Analytical Chemistry
Background:
- Bisphenol A (BPA) is a significant endocrine disrupting chemical (EDC) of environmental and human health concern.
- Existing methods for BPA detection are often complex and lack stable bioreceptors or signal generators.
- There is a need for simple, sensitive, and selective methods for monitoring BPA exposure.
Purpose of the Study:
- To develop a rapid, sensitive, and selective nucleic acid-based method for detecting bisphenol A (BPA).
- To address the limitations of current BPA detection techniques by utilizing a novel DNA aptamer and DNAzyme system.
Main Methods:
- A chemiluminescence (CL) assay was designed using a split ssDNA aptamer and a peroxidase-like ssDNAzyme.
- The anti-BPA aptamer incorporated one part of the split DNAzyme, with the other part serving as a complementary bait sequence.
- BPA presence was detected by observing the inhibition of DNAzyme activity and subsequent reduction in CL signal.
Main Results:
- The developed method achieved sensitive detection of BPA down to 5 nM.
- The assay demonstrated a broad dynamic range spanning five orders of magnitude.
- High selectivity for BPA was observed, distinguishing it from structurally similar EDCs.
Conclusions:
- This nucleic acid-based chemiluminescence method provides a simple, rapid, and sensitive platform for BPA detection.
- The developed aptamer-DNAzyme system is a promising tool for environmental monitoring of EDCs.
- Further development of aptamer-based detection strategies can enhance the study of EDC impacts.
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