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A sensitive ratiometric fluorescent probe for quantitive detection and imaging of alkaline phosphatase in living
Congcong Gao1, Shunping Zang1, Longxue Nie1
1Key Laboratory of Cluster Science of Ministry of Education, Beijing Key Laboratory of Photoelectronic/Electrophotonic Conversion Materials, School of Chemistry, Beijing Institute of Technology, Beijing, 100081, PR China.
Abstract:
We report a sensitive fluorescence probe Ratio-ALP for in vivo ratiometric imaging of alkaline phosphatase (ALP). The probe was designed by conjugating a naphthalimide with a phosphate group as the responsive moiety for enzymatic reactions. The fluorescence intensity ratio displayed a linear relationship against the concentration of ALP in the concentration range from 0 to 200 U/L, its LOD is as low as 0.25 U/L. Ratio-ALP has good selectivity and sensitivity, high cell permeability, and low cytotoxicity. Furthermore, probe Ratio-ALP was also successfully applied to image endogenous ALP activity in living cells.
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