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Significance of Tsukushi in lung cancer

Tatsuya Yamada1, Kunimasa Ohta2, Yamato Motooka1

  • 1Departments of Pathology and Experimental Medicine, Honjo 1-1-1, Chuo-ku, Kumamoto 860-8556, Japan; Thoracic Surgery, Honjo 1-1-1, Chuo-ku, Kumamoto 860-8556, Japan.

Lung Cancer (Amsterdam, Netherlands)
|April 28, 2019
PubMed
Abstract

Insights

Tsukushi (TSK) is highly expressed in lung cancer and promotes cell proliferation and epithelial-mesenchymal transition (EMT). TSK may be a key regulator in the lung cancer microenvironment.

Area of Science:

  • Oncology
  • Molecular Biology
  • Proteoglycan Research

Background:

  • Tsukushi (TSK) is a small leucine-rich repeat proteoglycan (SLRP) family member with known developmental roles.
  • The function of TSK in cancer, particularly lung cancer, has not been previously elucidated.

Purpose of the Study:

  • To investigate the biological significance and role of Tsukushi (TSK) in lung cancer.
  • To determine if TSK expression correlates with lung cancer progression and affects key cellular processes.

Main Methods:

  • TSK expression was analyzed in lung cancer tissues and cell lines using immunohistochemistry and RT-PCR.
  • Functional studies involved modifying TSK expression in a human lung adenocarcinoma cell line (H1975) and performing RNA sequencing, Gene Ontology analysis, Western blotting, cell counting, invasion assays, and xenograft studies.

Main Results:

  • TSK exhibited high expression in lung cancer tissues and cell lines compared to other SLRPs.
  • Altering TSK levels in H1975 cells affected molecules associated with epithelial-mesenchymal transition (EMT), including cadherin-1, snail1, snail2, and vimentin.
  • Cell counting and xenograft assays demonstrated that TSK promotes cell proliferation.

Conclusions:

  • TSK is significantly upregulated in lung cancer and plays a crucial role in regulating EMT and proliferation.
  • TSK may act as a key coordinator of multiple signaling pathways and a significant structural component within the lung cancer microenvironment.
Keywords:
Cell proliferationLung cancerSmall leucine-rich repeat proteoglycanTsukushiepithelial–mesenchymal transition

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