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Hhip inhibits proliferation and promotes differentiation of adipocytes through suppressing hedgehog signaling pathway

Haiyan Wei1, Jingjing Li1, Shengjie Shi1

  • 1Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, 712100, China; Laboratory of Animal Fat Deposition and Muscle Development, College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, 712100, China.

Biochemical and Biophysical Research Communications
|April 28, 2019
PubMed

Insights

Hedgehog interacting protein (Hhip) inhibits adipocyte proliferation and promotes differentiation, impacting lipid metabolism and offering a potential target for obesity prevention.

Area of Science:

  • Cell Biology
  • Metabolic Disease Research
  • Molecular Biology

Background:

  • Adipogenesis is key to body fat mass and lipid metabolism, with implications for obesity.
  • The Hedgehog interacting protein (Hhip) is part of the Hedgehog (Hh) signaling pathway, but its role in adipogenesis is unclear.

Purpose of the Study:

  • To investigate the physiological functions of Hhip in adipogenesis and lipid deposition.
  • To explore Hhip's potential as a therapeutic target for obesity.

Main Methods:

  • Analyzing Hhip levels during porcine adipose tissue development.
  • Treating cells with recombinant Hhip (rHhip) to assess effects on proliferation and differentiation.
  • Measuring gene expression (Glut4, PPARγ) and lipid accumulation.

Main Results:

  • Hhip levels were downregulated during porcine adipose tissue development.
  • rHhip inhibited cell proliferation by downregulating cell cycle genes.
  • rHhip promoted adipocyte differentiation, increasing lipid accumulation and upregulating Glut4 and PPARγ expression via canonical Hh signaling.

Conclusions:

  • Hhip plays a critical role in regulating adipocyte proliferation and differentiation.
  • Hhip represents a potential novel target for the prevention of obesity.

Keywords:
AdipocytesDifferentiationHedgehog signaling pathwayHhipProliferation

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