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Related Concept Videos

Transcription01:10

Transcription

155.9K
Overview
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...
155.9K
Transcription Factors02:16

Transcription Factors

82.3K
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
82.3K
Eukaryotic Transcription Activators02:42

Eukaryotic Transcription Activators

12.6K
Transcription activators are proteins that promote the transcription of genes from DNA to RNA. In most cases, these proteins contain two separate domains ‒ a domain that binds to DNA and a domain for activating transcription; however, in some cases, a single domain is responsible for both binding and activation of transcription, as seen in the glucocorticoid receptor and MyoD.
The binding domains are capable of recognizing and interacting with regulatory sequences on the DNA. These...
12.6K
Master Transcription Regulators02:23

Master Transcription Regulators

7.7K
Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
7.7K
Transcription Elongation Factors02:35

Transcription Elongation Factors

13.5K
Transcription elongation is a dynamic process that alters depending upon the sequence heterogeneity of the DNA being transcribed. Hence, it is not surprising that the elongation complex's composition also varies along the way while transcribing a gene.
The transcription elongation is regulated via pausing of RNA polymerase on several occasions during transcription. In bacteria, these halts are necessary because the transcription of DNA into mRNA is coupled to the translation of that mRNA...
13.5K
Prokaryotic Transcriptional Activators and Repressors01:58

Prokaryotic Transcriptional Activators and Repressors

25.2K
The organization of prokaryotic genes in their genome is notably different from that of eukaryotes. Prokaryotic genes are organized, such that the genes for proteins involved in the same biochemical process or function are located together in groups. This group of genes, along with their regulatory elements, are collectively known as an operon. The functional genes in an operon are transcribed together to give a single strand of mRNA known as polycistronic mRNA.
Transcription of prokaryotic...
25.2K

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Related Experiment Video

Updated: Jan 25, 2026

Single Cell Multiplex Reverse Transcription Polymerase Chain Reaction After Patch-clamp
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Single-Cell Tagged Reverse Transcription (STRT-Seq).

Kedar Nath Natarajan1

  • 1Functional Genomics and Metabolism Unit, Danish Institute of Advanced Study (D-IAS), University of Southern Denmark, Odense, Denmark. knn@bmb.sdu.dk.

Methods in Molecular Biology (Clifton, N.J.)
|April 28, 2019
PubMed
Summary
This summary is machine-generated.

This study details the Single-cell tagged reverse transcription sequencing on the Fluidigm C1 platform (STRT-C1) method. This approach enables accurate, sensitive, and molecular counting of transcripts for individual cells.

Keywords:
5′ Tag countingFluidigm C1MicrofluidicsSTRT-C1Single-cell tagged reverse transcriptionUMIsscRNA-seq

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Area of Science:

  • Molecular Biology
  • Genomics
  • Biotechnology

Background:

  • Single-cell RNA sequencing (scRNA-seq) is crucial for understanding cellular heterogeneity.
  • Early methods like STRT-seq focused on 5' tag counting for transcript profiling.

Purpose of the Study:

  • To describe the STRT-C1 method for single-cell transcriptomics.
  • To outline the workflow for capturing and preparing single-cell libraries.

Main Methods:

  • Utilizing the Fluidigm C1 microfluidics platform for cell capture.
  • Performing cDNA synthesis and library preparation for Illumina sequencing.
  • Employing 5' tag counting for transcript quantification.

Main Results:

  • Demonstrates a flexible scRNA-seq approach (STRT-C1).
  • Enables accurate and sensitive molecular counting of transcripts at the single-cell level.
  • Successfully prepares libraries for high-throughput sequencing.

Conclusions:

  • STRT-C1 is a robust method for single-cell transcriptomic analysis.
  • The described protocol facilitates detailed profiling of individual cells.
  • STRT-C1 offers a valuable tool for diverse biological research applications.