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Updated: Jan 24, 2026
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Ultralow Input Genome Sequencing Library Preparation from a Single Tardigrade Specimen
Published on: July 15, 2018
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Sequencing the Genomes of Single Cells.
Veronica Gonzalez-Pena1,2, Charles Gawad3,4
1Department of Oncology, St. Jude Children's Research Hospital, Memphis, TN, USA.
Methods in Molecular Biology (Clifton, N.J.)
|April 28, 2019
Summary
Single-cell genome sequencing reveals rare genetic changes in tissues. This guide details challenging experimental steps for high-quality data, including cell isolation and whole-genome amplification.
Area of Science:
- Genomics
- Molecular Biology
- Biotechnology
Background:
- Single-cell genome sequencing (scGS) is crucial for detecting low-frequency genetic alterations in complex tissues.
- Existing experimental protocols for scGS are technically demanding and complex.
Purpose of the Study:
- To provide a detailed protocol for performing single-cell genome sequencing.
- To enable researchers to obtain high-quality scGS data from complex tissues.
Main Methods:
- Tissue dissociation and single-cell isolation techniques.
- Whole-genome amplification (WGA) and sequencing library preparation.
- Optional target enrichment strategies for focused genomic analysis.
Main Results:
- High-quality single-cell genome sequencing data.
- Successful detection of low-frequency genetic alterations.
- Reproducible methodology for scGS.
Conclusions:
- This comprehensive protocol addresses the technical challenges of scGS.
- It facilitates the reliable analysis of genetic variations at the single-cell level.
- Enables deeper insights into tissue heterogeneity and disease mechanisms.
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