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Related Experiment Video

Updated: Jan 25, 2026

Ex vivo Live Imaging of Single Cell Divisions in Mouse Neuroepithelium
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Published on: April 30, 2013

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Single-Cell Live Imaging.

Toru Hiratsuka1, Naoki Komatsu2

  • 1Centre for Stem Cells and Regenerative Medicine, King's College London, Guy's Hospital, London, UK. toru.hiratsuka@kcl.ac.uk.

Methods in Molecular Biology (Clifton, N.J.)
|April 28, 2019
PubMed
Summary

Advanced fluorescence microscopy captures 5D live cell imaging for detailed cellular analysis. This technology offers new insights into physiological and pathological cell behaviors, including cancer drug responses.

Keywords:
2D/3D-cultured cell observationFluorescent probesLiving mouse observationMicroscopySingle cell imaging

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Area of Science:

  • Biophysics
  • Cell Biology
  • Microscopy

Background:

  • High-throughput 5D (X, Y, Z, T, Color) fluorescence microscopy enables detailed imaging of various biological samples.
  • This technology provides single-cell resolution for complex biological systems like cultured cells, spheroids, organoids, and living tissues.
  • Understanding heterogeneous cell phenotypes in physiological and pathological conditions, such as cancer cell drug responses, is crucial.

Purpose of the Study:

  • To provide an overview of advanced fluorescence microscopy applications for capturing live cell events.
  • To demonstrate the utility of 5D live imaging across diverse biological targets.
  • To present proof-of-concept examples for both 2D and 3D live imaging.

Main Methods:

  • Utilizing high-throughput 5D fluorescence microscopy for live cell imaging.
  • Implementing FRET-based time-lapse imaging for 2D cultured cell studies.
  • Developing and applying a 3D tissue imaging protocol, including live imaging of mouse skin.

Main Results:

  • Demonstrated the capability of 5D microscopy to capture dynamic cellular processes in various models.
  • Successfully applied FRET imaging to visualize molecular interactions in live cultured cells over time.
  • Established a robust protocol for high-resolution live imaging of intact biological tissues, exemplified by mouse skin.

Conclusions:

  • 5D fluorescence microscopy is a powerful tool for advancing cell biology and understanding complex biological phenomena.
  • Live imaging techniques offer unprecedented insights into cellular dynamics and responses in both normal and disease states.
  • The presented methods provide valuable protocols for researchers studying cellular behavior in diverse biological contexts.