Related Experiment Video

Updated: Jan 25, 2026

Purification of Endogenous Drosophila Transient Receptor Potential Channels
08:39

Purification of Endogenous Drosophila Transient Receptor Potential Channels

Published on: December 28, 2021

2.1K

Expression, Purification, and Crystallization of the Transient Receptor Potential Channel TRPV6

Appu K Singh1, Luke L McGoldrick1,2, Alexander I Sobolevsky3

  • 1Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY, USA.

Methods in Molecular Biology (Clifton, N.J.)
|April 28, 2019
PubMed

Insights

Transient receptor potential (TRP) channels, like TRPV6, are crucial for cellular functions and disease. This study details methods for their structural analysis, aiding drug discovery for diseases including cancer.

Area of Science:

  • Structural biology
  • Membrane protein biophysics
  • Ion channel research

Background:

  • Transient receptor potential (TRP) channels are vital sensory transducers implicated in diseases like cancer.
  • Their large size and dynamic nature present significant challenges for structural studies.
  • TRP channels are key drug targets due to their role in numerous pathologies.

Purpose of the Study:

  • To present a methodology for the structural study of TRPV6, a calcium-selective TRP channel.
  • To provide a detailed protocol for expression, purification, and crystallization of TRPV6.
  • To enable structural determination of other membrane proteins, including TRP channel family members.

Main Methods:

  • Expression and purification of TRPV6 protein.
  • Crystallization techniques for membrane proteins.
  • Methodology applicable to diverse TRP channel family members.

Main Results:

  • A robust protocol for TRPV6 structural studies was established.
  • The methodology facilitates structural determination of challenging membrane proteins.
  • The presented approach is adaptable for various TRP channels.

Conclusions:

  • The developed methodology is effective for structural analysis of TRPV6.
  • This work provides a foundation for understanding TRP channel structures and functions.
  • The protocol can be extended to other membrane proteins, advancing structural biology.
Keywords:
BaculovirusHEK 293 cellsIon channelMembrane proteinProtein crystallizationProtein expressionProtein purificationProtein structureSf9 cellsTRP channelTransient receptor potential channelX-ray crystallography

More Related Videos

Expression and Purification of Mammalian Bestrophin Ion Channels08:12

Expression and Purification of Mammalian Bestrophin Ion Channels

Published on: August 2, 2018

8.8K
Controllable Ion Channel Expression through Inducible Transient Transfection10:00

Controllable Ion Channel Expression through Inducible Transient Transfection

Published on: February 17, 2017

9.8K

Related Concept Videos

Ion Channels01:19

Ion Channels

The movement of ions like sodium, potassium, and calcium into and out of the cell is essential to maintain the electrochemical gradient in living cells. The ion channels—a class of membrane transport proteins—help maintain this ionic gradient for the smooth functioning of physiological activities such as maintaining cell size and volume, conducting nerve impulses, and gas and nutrient exchange.
Ion channels are specialized integral membrane proteins on the plasma membrane that allow...
91.2K
Ligand-Gated Ion Channel Receptor: Gating Mechanism01:30

Ligand-Gated Ion Channel Receptor: Gating Mechanism

Ligand-gated ion channels are transmembrane proteins that play a vital role in intercellular communication and functions of the nervous system. They allow the influx of ions across the membrane once the neurotransmitter binds, allowing the subsequent transmission of electrical excitation across the neurons. Other ligand-gated ion channels, like the γ-aminobutyric acid (GABA) receptor, permit anions like chloride into the cells on the binding of the GABA molecule. Their entry into the cell...
3.9K
Ionic Crystal Structures02:42

Ionic Crystal Structures

Ionic crystals consist of two or more different kinds of ions that usually have different sizes. The packing of these ions into a crystal structure is more complex than the packing of metal atoms that are the same size.
Most monatomic ions behave as charged spheres, and their attraction for ions of opposite charge is the same in every direction. Consequently, stable structures for ionic compounds result (1) when ions of one charge are surrounded by as many ions as possible of the opposite...
16.9K
Internal Receptors01:31

Internal Receptors

Many cellular signals are hydrophilic and therefore cannot pass through the plasma membrane. However, small or hydrophobic signaling molecules can cross the hydrophobic core of the plasma membrane and bind to internal, or intracellular, receptors that reside within the cell. Many mammalian steroid hormones use this mechanism of cell signaling, as does nitric oxide (NO) gas.
74.4K
Crystal Growth: Principles of Crystallization01:25

Crystal Growth: Principles of Crystallization

Crystallization is a phase transformation process in which crystals are precipitated from a supersaturated solution or formed from other sources. During crystallization, atoms or molecules arrange themselves into a well-defined, rigid crystal lattice to minimize energy.
Initiating crystallization involves manipulating the concentration of the solute and the temperature of the solution. Since crystal growth occurs when the ratio of concentration and solubility of the solute in the solvent...
4.9K
Crystal Field Theory - Octahedral Complexes02:58

Crystal Field Theory - Octahedral Complexes

Crystal Field Theory
To explain the observed behavior of transition metal complexes (such as colors), a model involving electrostatic interactions between the electrons from the ligands and the electrons in the unhybridized d orbitals of the central metal atom has been developed. This electrostatic model is crystal field theory (CFT). It helps to understand, interpret, and predict the colors, magnetic behavior, and some structures of coordination compounds of transition metals.
CFT focuses on...
30.8K