Related Experiment Video

Updated: Jan 25, 2026

Global Gene Expression Analysis Using a Zebrafish Oligonucleotide Microarray Platform
13:14

Global Gene Expression Analysis Using a Zebrafish Oligonucleotide Microarray Platform

Published on: August 10, 2009

12.2K

Integrated analysis of global gene and microRNA expression profiling associated with aplastic anaemia

Sarmistha Adhikari1, Paramita Mandal1

  • 1Biomedical Genetics Laboratory, Department of Zoology, The University of Burdwan, West Bengal, India.

Life Sciences
|April 28, 2019
PubMed
Abstract

Insights

Researchers identified miR-1202 upregulation in aplastic anaemia patients. Inhibiting miR-1202 may reverse disease phenotype by upregulating target genes like RAPGEF5 and MANEA.

Area of Science:

  • Hematology
  • Molecular Biology
  • Genetics

Background:

  • Aplastic anaemia is a rare bone marrow disorder.
  • MicroRNA (miRNA) dysregulation is implicated in various diseases.
  • miRNA-targeted therapies show promise for hematological disorders.

Purpose of the Study:

  • To identify differentially expressed miRNAs in aplastic anaemia patients.
  • To investigate the role of miR-1202 in aplastic anaemia pathogenesis.
  • To explore potential therapeutic strategies targeting miR-1202.

Main Methods:

  • Global miRNA and gene expression profiling using Affymetrix microarrays.
  • Analysis of publicly available datasets (NCBI GEO: GSE3807, GSE82095).
  • Bioinformatic prediction of miRNA-target gene interactions.

Main Results:

  • miR-1202 was significantly upregulated in aplastic anaemia patients.
  • RAPGEF5 and MANEA genes were downregulated in aplastic anaemia and upregulated post-therapy.
  • miR-1202 potentially targets RAPGEF5 and MANEA, suggesting a regulatory role.

Conclusions:

  • miR-1202 upregulation contributes to aplastic anaemia phenotype.
  • Inhibiting miR-1202 could be a therapeutic strategy.
  • Upregulation of target genes (RAPGEF5, MANEA) via inhibition may reverse disease characteristics.
Keywords:
Aplastic anaemiaGene regulationT cellsmiR-1202

More Related Videos

Profiling of Estrogen-regulated MicroRNAs in Breast Cancer Cells16:24

Profiling of Estrogen-regulated MicroRNAs in Breast Cancer Cells

Published on: February 21, 2014

20.7K
Isolation and Profiling of MicroRNA-containing Exosomes from Human Bile06:59

Isolation and Profiling of MicroRNA-containing Exosomes from Human Bile

Published on: June 13, 2016

10.8K

Related Concept Videos

MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
24.1K
What is Gene Expression?01:42

What is Gene Expression?

Overview
Gene expression is the process in which DNA directs the synthesis of functional products, that is, proteins. Cells can regulate gene expression at various stages. It allows organisms to generate different cell types and enables cells to adapt to internal and external factors.
Genetic Information Flows from DNA to RNA to Protein
A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is made up of nucleotides and proteins consist of amino...
195.2K
What is Gene Expression?01:36

What is Gene Expression?

A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is comprised  of nucleotides and proteins are comprised of amino acids, a mediator is required to convert the information encoded in DNA into proteins. This mediator is the messenger RNA (mRNA). mRNA copies the blueprint from DNA by a process called transcription. In eukaryotes, transcription occurs in the nucleus by complementary base-pairing with the DNA template. The mRNA is then...
11.2K
Cell Specific Gene Expression01:58

Cell Specific Gene Expression

Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
16.3K
Cell Specific Gene Expression01:58

Cell Specific Gene Expression

5.4K
Chromatin Position Affects Gene Expression02:35

Chromatin Position Affects Gene Expression

Chromatin is the massive complex of DNA and proteins packaged inside the nucleus. The complexity of chromatin folding and how it is packaged inside the nucleus greatly influences  access to genetic information. Generally, the nucleus' periphery is considered transcriptionally repressive, while the cell's interior is considered a transcriptionally active area. 
Topologically Associated Domains (TADs)
The 3-dimensional positioning of chromatin in the nucleus influences the...
24.7K