Shedding light on the dark matter of the biomolecular structural universe: Progress in RNA 3D structure prediction

Structured RNA plays many functionally relevant roles in molecular life. Structural information, while required to understand the functional cycles in detail, is challenging to gather. Computational methods promise to complement experimental efforts by predicting three-dimensional RNA models. Here, we provide a concise view of the state of the art methodologies with a focus on the strengths and the weaknesses of the different approaches. Furthermore, we analyzed the recent developments regarding the use of coevolutionary information and how it can boost the prediction performances. We finally discuss some open perspectives and challenges for the near future in the RNA structural stability field.