Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Olfaction01:25

Olfaction

48.4K
The sense of smell is achieved through the activities of the olfactory system. It starts when an airborne odorant enters the nasal cavity and reaches olfactory epithelium (OE). The OE is protected by a thin layer of mucus, which also serves the purpose of dissolving more complex compounds into simpler chemical odorants. The size of the OE and the density of sensory neurons varies among species; in humans, the OE is only about 9-10 cm2.
The olfactory receptors are embedded in the cilia of the...
48.4K
Tandem Mass Spectrometry01:21

Tandem Mass Spectrometry

2.4K
Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...
2.4K
Mass Spectrometry: Overview01:19

Mass Spectrometry: Overview

8.4K
Mass spectrometry is an analytical technique used to determine the molecular mass and molecular formula of a compound. The basic principle of mass spectrometry is to generate ions from the analyte molecule and measure these ion abundances against their molecular mass. One common type of ionization, known as electron ionization or EI, bombards the analyte molecules in the gas phase with high-energy electron beams. The electron beams displace an electron from the molecule and leave behind a...
8.4K
Mass Spectrometry of Amines01:15

Mass Spectrometry of Amines

5.3K
In mass spectroscopy, amines undergo fragmentation to give parent ions with odd molecule weights. This observed mass spectrum follows the nitrogen rule; a molecule with an odd number of nitrogen atoms produces a molecular ion with an odd molecular weight. Amines undergo fragmentation through α cleavage, producing nitrogen-containing cations—iminium ions—and alkyl radicals. Mass spectra of aromatic and cyclic aliphatic amines exhibit strong molecular ion peaks, but acyclic...
5.3K
Mass Spectrometry: Isotope Effect01:13

Mass Spectrometry: Isotope Effect

4.0K
Most elements exist in nature as a mixture of isotopes. The isotopes differ in weight due to their respective number of neutrons. The molecular weight of a molecule is different depending on the specific isotope of its elements involved. As a result, the mass spectrum of the molecule exhibits peaks from the same fragment at multiple positions. The positions of these mass signals depend on the mass differences between isotopes. Furthermore, the intensity of these signals is dependent on the...
4.0K
MALDI-TOF Mass Spectrometry01:19

MALDI-TOF Mass Spectrometry

6.6K
Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...
6.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Facile Synthesis of Primary Amines via Nitrogen Fixation in Charged Microdroplets.

Nature communications·2026
Same author

Differentiation of disaccharide isomers <i>via</i> diagnostic-ion-weighted spectral library searching using data from tandem MS analysis of chloride adducts.

The Analyst·2026
Same author

Enzyme-centric chemoproteomics reveals isomer-specific S-acylation modification networks.

Nature communications·2026
Same author

A Six-Membered Concerted Mechanism for CO<sub>2</sub> Capture by Amines Studied under Charged Microdroplet Reaction Conditions.

Analytical chemistry·2026
Same author

A paper-based immunoassay with signal amplification for the sensitive detection of nucleocapsid protein toward the diagnosis of long COVID.

The Analyst·2026
Same author

Chemoselective Tagging of Protein Methacrylation.

Journal of the American Chemical Society·2025

Related Experiment Video

Updated: Jan 25, 2026

Visualization of Ambient Mass Spectrometry with the Use of Schlieren Photography
06:49

Visualization of Ambient Mass Spectrometry with the Use of Schlieren Photography

Published on: June 20, 2016

8.7K

Reactive Olfaction Ambient Mass Spectrometry.

Dmytro S Kulyk1, Taghi Sahraeian1, Qiongqiong Wan1

  • 1Department of Chemistry and Biochemistry , The Ohio State University , 100 W. 18th Avenue , Columbus , Ohio 43210 , United States.

Analytical Chemistry
|April 30, 2019
PubMed
Summary

A novel nano-atmospheric pressure chemical ionization (nAPCI) source enables direct mass spectrometry analysis of low-volatility organic compounds without physical contact, revolutionizing chemical analysis.

More Related Videos

Applications of the Single-probe: Mass Spectrometry Imaging and Single Cell Analysis under Ambient Conditions
15:00

Applications of the Single-probe: Mass Spectrometry Imaging and Single Cell Analysis under Ambient Conditions

Published on: June 14, 2016

11.2K
Semi-Quantitative Analysis of Peptidoglycan by Liquid Chromatography Mass Spectrometry and Bioinformatics
09:09

Semi-Quantitative Analysis of Peptidoglycan by Liquid Chromatography Mass Spectrometry and Bioinformatics

Published on: October 13, 2020

5.1K

Related Experiment Videos

Last Updated: Jan 25, 2026

Visualization of Ambient Mass Spectrometry with the Use of Schlieren Photography
06:49

Visualization of Ambient Mass Spectrometry with the Use of Schlieren Photography

Published on: June 20, 2016

8.7K
Applications of the Single-probe: Mass Spectrometry Imaging and Single Cell Analysis under Ambient Conditions
15:00

Applications of the Single-probe: Mass Spectrometry Imaging and Single Cell Analysis under Ambient Conditions

Published on: June 14, 2016

11.2K
Semi-Quantitative Analysis of Peptidoglycan by Liquid Chromatography Mass Spectrometry and Bioinformatics
09:09

Semi-Quantitative Analysis of Peptidoglycan by Liquid Chromatography Mass Spectrometry and Bioinformatics

Published on: October 13, 2020

5.1K

Area of Science:

  • Analytical Chemistry
  • Mass Spectrometry
  • Chemical Ionization

Background:

  • Direct analysis of organic compounds with negligible vapor pressure (VP) is challenging using traditional methods.
  • Existing techniques often require sample preparation or physical contact, limiting their applicability.
  • Atmospheric pressure chemical ionization (APCI) typically requires analytes with sufficient volatility.

Purpose of the Study:

  • To develop a new contained nano-atmospheric pressure chemical ionization (nAPCI) source for analyzing low-volatility organic compounds.
  • To enable direct mass spectrometry analysis of complex mixtures without physical contact.
  • To characterize the ionization of diverse polar and nonpolar compounds under ambient conditions.

Main Methods:

  • Development of a contained nAPCI source utilizing corona discharge for ionization.
  • Reactive olfaction process involving electrostatic charge induction and particle liberation.
  • Direct mass spectrometry analysis of various samples including biological fluids, perfumes, and beverages.
  • Quantification of analytes at low concentrations (ng/mL and pg/mL).

Main Results:

  • Successful chemical ionization of organic compounds with extremely low vapor pressures (e.g., carminic acid, vitamin D2).
  • Detection of cocaine in serum (1 ng/mL) and caffeine in urine (200 pg/mL).
  • Differentiation of chemical compositions in complex mixtures like perfumes and beverages.
  • Characterization of ion types for 78 organic compounds.
  • Achieved sample consumption rates below nmol/min.

Conclusions:

  • The contained nAPCI source provides a versatile and sensitive platform for direct mass spectrometry analysis of low-volatility compounds.
  • This method overcomes limitations of traditional techniques, enabling analysis of previously inaccessible samples.
  • The nAPCI source demonstrates broad applicability in fields requiring rapid chemical analysis of complex mixtures.