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In vitro analysis of genetically distinct Chlamydia pecorum isolates reveals key growth differences in mammalian

Md Mominul Islam1, Martina Jelocnik1, Susan Anstey1

  • 1Genecology Research Centre, Faculty of Science, Health, Education and Engineering, University of the Sunshine Coast, Sippy Downs, Australia.

Veterinary Microbiology
|April 30, 2019
PubMed

Insights

Distinct Chlamydia (C.) pecorum strains show varied growth in different animal cells, impacting our understanding of C. pecorum pathogenesis and virulence factors.

Area of Science:

  • Veterinary Microbiology
  • Bacterial Pathogenesis
  • Cell Biology

Background:

  • Chlamydia (C.) pecorum is a genetically diverse bacterium causing varied diseases in animals.
  • Limited data exist on C. pecorum pathogenesis and the impact of genetic diversity on disease outcomes.
  • Understanding C. pecorum virulence factors requires more in vitro and in vivo data.

Purpose of the Study:

  • To investigate if distinct C. pecorum isolates exhibit different in vitro growth phenotypes.
  • To compare the growth of various C. pecorum isolates in different mammalian epithelial and immune cells.
  • To identify potential C. pecorum virulence factors linked to observed phenotypic differences.

Main Methods:

  • In vitro culture of five distinct C. pecorum isolates (IPA, E58, 1710S, W73, JP-1-751).
  • Growth assessment in McCoy cells, bovine peripheral neutrophil granulocytes, and bovine monocyte-derived macrophages.
  • Analysis of inclusion size and lag phase as indicators of growth phenotype.

Main Results:

  • Isolates W73 and JP-1-751 showed shorter lag phases in McCoy cells.
  • The plasmid-free E58 isolate formed significantly smaller inclusions.
  • Bovine and ovine isolates grew faster in bovine cells than the porcine isolate (1710S).
  • Neutrophils supported infection but not complete development; macrophages supported multiplication for most isolates.

Conclusions:

  • Genetically distinct C. pecorum isolates display significant phenotypic differences in vitro.
  • These differences in growth and replication provide insights into C. pecorum pathogenesis.
  • The findings aid in identifying C. pecorum virulence factors and understanding host-pathogen interactions.
Keywords:
Chlamydia pecorumDevelopmental cycleGenetically distinctIn vitro growth

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