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Ultrasensitive Optical Chemosensor for Cu(II) Detection.

Sayed M Saleh1,2, Reham Ali1,3, Fahad Alminderej1

  • 1Chemistry Department, Science College, Qassim University, Buraidah, Saudi Arabia.

International Journal of Analytical Chemistry
|April 30, 2019
PubMed
Summary

A new optical chemosensor, BDC, detects copper ions (Cu(II)) with high selectivity and sensitivity. This fluorescent probe offers a fast and ultrasensitive method for tracing Cu(II) in biological systems.

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Area of Science:

  • Analytical Chemistry
  • Materials Science
  • Biochemistry

Background:

  • Copper(II) ions (Cu(II)) play crucial roles in biological processes.
  • Accurate detection of Cu(II) is vital for understanding its physiological functions.
  • Existing detection methods may lack selectivity, sensitivity, or applicability in biological conditions.

Purpose of the Study:

  • To design and synthesize a novel optical chemosensor, BDC.
  • To evaluate the chemosensor's performance for Cu(II) detection.
  • To explore the potential of BDC for biological applications.

Main Methods:

  • Synthesis of 2,6-Bis(4-dimethylaminophenyl)-4-(dicyanomethylene)-cyclohexane-1,1-dicarbo-nitrile (BDC).
  • Spectroscopic analysis (fluorescence and UV-vis absorption) in methanol-HEPES buffer (pH 7.2).
  • Selectivity and sensitivity tests with various metal ions and determination of the limit of detection (LOD).

Main Results:

  • BDC exhibits a large Stokes shift (approx. 170 nm) and selective fluorescence quenching upon binding with Cu(II).
  • The chemosensor demonstrates high selectivity for Cu(II) over other cations.
  • A low limit of detection (LOD) of 2.3 x 10-7 M and a binding constant (Kbind) of 7.16 x 104 M-1 were achieved.
  • The detection mechanism involves a 1:1 chelation process, confirmed by spectral changes.

Conclusions:

  • BDC is a highly selective and sensitive fluorescent chemosensor for Cu(II) detection.
  • The probe operates effectively at physiological pH (7.2), making it suitable for biological studies.
  • BDC offers a promising, ultrasensitive, and fast method for investigating the biological roles of Cu(II) in living cells.