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Updated: Jan 25, 2026
In Vitro Synthesis of Modified mRNA for Induction of Protein Expression in Human Cells
Published on: November 13, 2014
[Deoxynivalenol induced mRNA expressions of inflammation and apoptosis in BeWo cells]
Miao Yu1, Wei Hou2, Liangkai Chen3
1Wuxi School of Medicine, Jiangnan University, Wuxi 214122, China.
Objective:
To explore the effects of deoxynivalenol-induced mRNA expressions of inflammation and apoptosis in BeWo cells.
Methods:
After been cultured and transferred, BeWo cells were seeded for the CCK-8 assay. Then incubated with DON in the appropriate doses and time according to the CCK-8 result. ELISA kit was used to measure the β-HCG levels in BeWo cells. qPCR was used to measure mRNA expressions of inflammation(IL-6, IL-8, TNF-α and Cox-2) and apoptosis(Caspase-3 and Bcl-xl) in BeWo cells.
Results:
Compared with the control group, IL-6, TNF-α and Cox-2 significantly increased in BeWo cells which were exposed in 50 nmol/L DON in 3, 12 and 24 h(P<0. 01), while IL-8 only significantly increased in 24 h(P<0. 01). Caspase-3 significantly decreased in 3, 12 and 24 h(P<0. 01), and Bcl-xl significantly increased in 24 h(P<0. 01).
Conclusion:
The change in the level of inflammation and apoptosis may be one of the critical molecular mechanisms for DON-induced embryotoxicity.
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