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Updated: Jan 25, 2026

High-fat Feeding Paradigm for Larval Zebrafish: Feeding, Live Imaging, and Quantification of Food Intake
Published on: October 27, 2016
Fine grinding or expanding of feed as pre-treatment for pelleting in dependence on dietary rapeseed expeller
Wendy Liermann1,2, Andreas Berk2, Liane Hüther2
1a Institute of Nutritional Physiology , "Oskar Kellner", Leibniz Institute for Farm Animal Biology , Dummerstorf , Germany.
Abstract:
Although commercial broiler feed is usually differently conditioned before pelleting, the nutritional consequences of fine grinding or expanding as pre-pelleting treatments are poorly defined so far. Therefore, the effects of these two pre-treatments on nutrient digestibility, broiler performance and digestive tract of broilers were tested. In order to investigate possible interactions between pre-treatments and diet composition two diets differing in rapeseed expeller proportion were tested in a two by two factorial design. Thus, four diets were designed including two diets containing 6% rapeseed expeller (RSE) which were pre-treated by fine grinding (6%FgP) or expanding (6%ExP), and two corresponding diets containing 12% RSE (12%FgP and 12%ExP). For the experiments, 864 male broilers were used. There was a significant diet-by-technical feed treatment (TFT) interaction in case of the digestibility of all considered crude nutrients (p < 0.05). Diet 6%ExP showed higher crude protein digestibility compared to other feeds (p < 0.001). The highest digestibility of organic matter, ether extract, crude fibre and N-free extractives achieved diet 12%FgP. Diets 6%ExP and 12%FgP showed higher N-corrected metabolisable energy content (p < 0.001). TFT affected daily feed intake (DFI) and body weight (BW) gain in a diet-dependent manner (p < 0.001). Feeding of 6%FgP enhanced DFI and BW gain compared to other feeds but 6%ExP reduced both parameters (p < 0.001). Weights of proventriculi and gizzards of animals fed 6%ExP were increased compared with 6%FgP (p < 0.01). In contrast, proventricular length in animals fed 6%FgP was increased compared with diet 6%ExP (p = 0.042). Moreover, animals fed 6%FgP had wider proventriculi than animals fed 12%FgP (p = 0.023). Feed 6%ExP increased proventricular weight compared to 12%ExP (p = 0.001). With regard to the strong relationships between diet and TFT no specific processing method can be recommended according to considered nutritional aspects. A marked prevention of proventricular dilatation due to pellet feeding could not be realised by various used TFT or feed formulations. Used amounts of RSE had no obvious adverse effects on considered nutritional aspects.
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