Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

B cell activation in multiple sclerosis.

M Sandberg, A I Levinson, B Zweiman

    Acta Neurologica Scandinavica
    |December 1, 1986
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Leukocyte activation and the asthmatic response.

    Immunology today·2014
    Same author

    The nexus between atopic disease and autoimmunity: a review of the epidemiological and mechanistic literature.

    Clinical and experimental immunology·2008
    Same author

    Coherence times of dressed states of a superconducting qubit under extreme driving.

    Physical review letters·2007
    Same author

    A first look at duloxetine (Cymbalta) in a postmortem laboratory.

    Journal of analytical toxicology·2006
    Same author

    Genetic variability of Campylobacter jejuni isolated from fresh and frozen broiler carcasses.

    Journal of applied microbiology·2006
    Same author

    Non-invasive monitoring of muscle blood perfusion by photoplethysmography: evaluation of a new application.

    Acta physiologica Scandinavica·2005

    A new microtechnique enables the study of limited cerebrospinal fluid (CSF) cells, revealing increased immunoglobulin-secreting cells (IgSC) and IgG in multiple sclerosis patients. This method aids functional studies with scarce cell samples.

    Area of Science:

    • Immunology
    • Neuroscience
    • Cell Biology

    Background:

    • Studying cerebrospinal fluid (CSF) cells is challenging due to limited cell numbers.
    • Understanding B cell responses in neurological conditions requires sensitive techniques.

    Purpose of the Study:

    • To establish a microtechnique for analyzing immunoglobulin-secreting cells (IgSC) from limited CSF samples.
    • To compare IgSC responses in CSF cells (CSFC) and peripheral blood mononuclear cells (PBMC).
    • To investigate in vivo activation of CSF cells in multiple sclerosis (MS) patients.

    Main Methods:

    • Developed a microculture technique for low-cell-number samples.
    • Quantified IgSC and secreted immunoglobulins (IgG) using pokeweed mitogen (PWM).
    • Assessed the effect of autologous peripheral blood mononuclear cells (PBMCM) on CSFC and PBMC cultures.

    Related Experiment Videos

    Main Results:

    • Dose-response and kinetic profiles of IgSC were similar for CSFC and PBMC.
    • Unstimulated CSFC from MS patients showed significantly higher IgSC numbers and IgG secretion than PBMC.
    • PWM stimulation increased IgSC and IgG in both CSFC and PBMC.
    • Autologous PBMCM augmented IgSC numbers and IgG secretion in both CSFC and PBMC cultures.

    Conclusions:

    • The microtechnique is effective for functional studies with limited CSF cells.
    • CSF cells from MS patients may exhibit in vivo activation.
    • The findings provide insights into B cell activity in the CSF of MS patients.