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Covalently Linked Protein Regulators

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Related Experiment Video

Updated: Jun 25, 2026

Monitoring Cell-autonomous Circadian Clock Rhythms of Gene Expression Using Luciferase Bioluminescence Reporters

Monitoring Cell-autonomous Circadian Clock Rhythms of Gene Expression Using Luciferase Bioluminescence Reporters

Published on: September 27, 2012

Circadian rhythms and proteomics: It's all about posttranslational modifications!

Daniel Mauvoisin¹

¹Institute of Bioengineering, School of Life Sciences, Ecole Polytechnique Fédérale de Lausanne, Lausanne, Switzerland.

Wiley Interdisciplinary Reviews. Systems Biology and Medicine

|April 30, 2019

Summary

Circadian proteomics reveals how protein modifications link the body's internal clock to metabolism. This research explores rhythmic gene expression at the protein level, impacting metabolic health.

Keywords:

cell signaling, circadian rhythms metabolism post-translational modifications proteomics

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Last Updated: Jun 25, 2026

Monitoring Cell-autonomous Circadian Clock Rhythms of Gene Expression Using Luciferase Bioluminescence Reporters

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Published on: September 27, 2012

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Parallel Measurement of Circadian Clock Gene Expression and Hormone Secretion in Human Primary Cell Cultures

Parallel Measurement of Circadian Clock Gene Expression and Hormone Secretion in Human Primary Cell Cultures

Published on: November 11, 2016

Area of Science:

Physiology
Biological Mechanisms
Proteomics

Background:

The circadian clock synchronizes physiology and behavior to daily cycles.
Peripheral clocks in organs like the liver regulate metabolic gene expression.
Metabolism influences the circadian clock through posttranslational modifications.

Purpose of the Study:

To review circadian gene expression studies at the protein level.
To discuss the role of posttranslational modifications in linking the circadian clock and metabolism.
To highlight advances in circadian proteomics.

Main Methods:

Analysis of protein expression and posttranslational modifications.
Review of existing literature on circadian gene expression at the protein level.
Application of mass spectrometry for large-scale proteomic studies.

Main Results:

The majority of protein-coding genes exhibit tissue-specific circadian rhythms.
Proteomics enables large-scale studies of circadian rhythms at cellular, subcellular, and posttranslational levels.
Cell signaling is highly relevant to circadian rhythms and metabolic health.

Conclusions:

Circadian proteomics offers new insights into molecular clock mechanisms.
Posttranslational modifications are crucial links between circadian rhythms and metabolic regulation.
Future proteomics studies will deepen the understanding of cell signaling in circadian biology.