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Potassium uptake systems of Candida krusei
Hana Elicharova1, Pavla Herynkova1, Olga Zimmermannova1
1Department of Membrane Transport, Institute of Physiology of the Czech Academy of Sciences, Prague, Czech Republic.
Yeast (Chichester, England)
|April 30, 2019
Summary
Candida krusei utilizes two TRK genes for potassium uptake. Only CkTrk1 effectively complements potassium import in yeast, while CkTrk2 shows mislocalization and no functional complementation, highlighting differences in their targeting mechanisms.
Area of Science:
- Mycology
- Molecular Biology
- Biochemistry
Background:
- Candida krusei is an important pathogenic yeast.
- Potassium uptake is crucial for yeast proliferation and cell function.
- TRK genes encode potassium uptake systems conserved in Saccharomycetales.
Purpose of the Study:
- To identify and characterize potassium uptake systems in Candida krusei.
- To investigate the functional complementation and localization of C. krusei TRK genes in Saccharomyces cerevisiae.
Main Methods:
- Bioinformatic analysis of sequenced C. krusei genomes.
- Heterologous expression of C. krusei TRK genes in S. cerevisiae.
- Functional complementation assays and protein localization studies.
Main Results:
- Two conserved TRK genes, CkTRK1 and CkTRK2, were identified in C. krusei.
- CkTrk1 successfully complemented the potassium uptake deficiency in S. cerevisiae and showed partial vacuolar membrane localization.
- CkTrk2 failed to complement the S. cerevisiae deficiency, indicating improper plasma membrane targeting.
Conclusions:
- CkTrk1 is a functional potassium transporter in C. krusei capable of complementing heterologous systems.
- CkTrk2 exhibits an unusual N-terminus and is not correctly targeted to the plasma membrane in S. cerevisiae, representing a novel finding for yeast TRK proteins.
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