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Creation of a Dense Transposon Insertion Library Using Bacterial Conjugation in Enterobacterial Strains Such As Escherichia Coli or Shigella flexneri
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Creation of a Dense Transposon Insertion Library Using Bacterial Conjugation in Enterobacterial Strains Such As Escherichia Coli or Shigella flexneri

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Plasmids of Shigella flexneri serotype 1c strain Y394 provide advantages to bacteria in the host

Pawan Parajuli1, Munazza I Rajput1, Naresh K Verma2

  • 1Division of Biomedical Science and Biochemistry, Research School of Biology, The Australian National University, Canberra, ACT, Australia.

BMC Microbiology
|May 1, 2019
PubMed
Abstract

Insights

Shigella flexneri serotype 1c strain Y394 possesses two plasmids. One plasmid (pINV-Y394) contains virulence genes, while the other (pNV-Y394) confers multidrug resistance, highlighting the pathogen's adaptability.

Area of Science:

  • Microbiology
  • Genomics
  • Pathogen Evolution

Background:

  • Shigella flexneri exhibits a complex genome with virulence factors acquired via mobile genetic elements.
  • Serotype 1c of S. flexneri is an emerging cause of bacillary dysentery in developing nations.

Purpose of the Study:

  • To determine and analyze the complete nucleotide sequences of two plasmids from S. flexneri serotype 1c strain Y394.
  • To understand the genetic basis of S. flexneri 1c pathogenicity and adaptability.

Main Methods:

  • Whole-genome sequencing of two S. flexneri Y394 plasmids.
  • Bioinformatic analysis of plasmid nucleotide sequences.
  • Identification and characterization of open reading frames (ORFs) and gene functions.

Main Results:

  • The invasive plasmid pINV-Y394 (221,293 bp) contains numerous insertion sequences (159 ORFs) and virulence-associated genes (97 ORFs).
  • The non-virulence plasmid pNV-Y394 (10,866 bp) carries multidrug resistance genes, including tetA, strA-strB, and sul2.
  • A significant portion of ORFs on pINV-Y394 were uncharacterized (72 ORFs).

Conclusions:

  • The analyzed plasmids are crucial for the fitness and host adaptation of S. flexneri Y394.
  • Pathogenic S. flexneri demonstrates niche adaptation, co-evolution with hosts, and response to environmental selection pressures.
Keywords:
Bacterial pathogenMultidrug resistancePlasmidsShigella flexneriVirulence

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