Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Phenotyping of human complement component C4, a class-III HLA antigen.

E Sim, S J Cross

    The Biochemical Journal
    |November 1, 1986
    PubMed
    Summary

    This study introduces a new method for identifying complement protein C4 variants. Digestion with carboxypeptidase B simplifies C4 allotype analysis, improving accuracy in human C4 phenotype determination.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    'Who Listens to the Listener, Who Cares for the Carer?' A Cross-Sectional Study of Social Connectedness and Sleep Experiences of Young Siblings of Neurodivergent People.

    Child: care, health and development·2024
    Same author

    Glial mechanisms underlying substance use disorders.

    The European journal of neuroscience·2018
    Same author

    Clinical Pharmacogenetics Implementation Consortium (CPIC) Guidelines for CYP2C19 and Voriconazole Therapy.

    Clinical pharmacology and therapeutics·2016
    Same author

    Human VEGF165-myoblasts produce concomitant angiogenesis/myogenesis in the regenerative heart.

    Molecular and cellular biochemistry·2016
    Same author

    Multivariate assessment of site of lingual nerve.

    The British journal of oral & maxillofacial surgery·2015
    Same author

    Arylamine N-acetyltransferases: from drug metabolism and pharmacogenetics to drug discovery.

    British journal of pharmacology·2014

    Area of Science:

    • Immunogenetics
    • Biochemistry
    • Molecular Biology

    Background:

    • The complement protein C4 is crucial in the immune system.
    • C4 is encoded by highly polymorphic genes (C4A and C4B) in the major histocompatibility complex.
    • Existing methods for identifying C4 allotypes using electrophoresis are complex due to overlapping banding patterns.

    Purpose of the Study:

    • To develop a more accurate and interpretable method for determining human C4 allotypes.
    • To elucidate the biochemical basis of the complex banding patterns observed in C4 phenotyping.

    Main Methods:

    • Plasma samples were digested with carboxypeptidase B to remove C-terminal basic amino acids.
    • The treated samples were then analyzed using agarose-gel electrophoresis.
    • C4 allotypes were identified by immunofixation with anti-C4 antibodies.

    Main Results:

    • Carboxypeptidase B digestion resulted in a single, sharp, and distinct band for each C4 allotype.
    • This simplification resolved the previously observed overlapping banding patterns.
    • The method allows for clear identification of individual C4 allotypes and their biochemical basis.

    Conclusions:

    • Digestion with carboxypeptidase B is an effective method for simplifying C4 allotype analysis.
    • This technique significantly improves the accuracy and interpretability of human C4 phenotyping.
    • The findings provide a clearer understanding of C4 polymorphism and its implications.

    Related Experiment Videos