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Related Experiment Video

Updated: Jan 25, 2026

Microfluidics-based High-throughput Circulating Tumor Cell Sorting and Single-cell Sequencing Technology
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Microfluidics-based High-throughput Circulating Tumor Cell Sorting and Single-cell Sequencing Technology

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A microfluidic platform for high-purity separating circulating tumor cells at the single-cell level.

Kun Wang1, Lin Zhou2, Simin Zhao3

  • 1State Key Laboratory of Transducer Technology, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, Shanghai 200050, China; University of Chinese Academy of Sciences, Beijing 100000, China.

Talanta
|May 1, 2019
PubMed
Summary

This study introduces a novel microchip for highly effective and selective isolation of circulating tumor cells (CTCs). The microchip significantly improves CTC detection and analysis, aiding in early cancer diagnosis and personalized treatment strategies.

Keywords:
CTCsHigh-purity isolationMicrovalvesOn-chip analysis

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Area of Science:

  • Biotechnology
  • Medical Diagnostics
  • Nanotechnology

Background:

  • Circulating tumor cells (CTCs) are crucial for metastasis but are rare and heterogeneous, challenging current isolation methods.
  • Early detection of CTCs improves patient prognosis and treatment planning.
  • Existing CTC isolation techniques lack sufficient efficacy and selectivity.

Purpose of the Study:

  • To develop an integrated microchip for efficient and selective isolation and analysis of CTCs.
  • To overcome the limitations of current CTC detection methods.
  • To enable on-chip analysis of CTCs for clinical applications.

Main Methods:

  • Design of a microchip with integrated trap, bypass, and release channels for cell isolation.
  • Utilizing cell size and immuno-phenotype for preferential capture and selective release of CTCs.
  • On-chip analysis of epithelial-mesenchymal transition markers on isolated CTCs.

Main Results:

  • Achieved an average recovery rate of 92.5% and purity of 94% for lung cancer cells.
  • Demonstrated significantly higher performance compared to anti-CD45 magnetic bead methods.
  • Successfully analyzed surface markers of epithelial-mesenchymal transition on isolated CTCs.

Conclusions:

  • The integrated microchip offers a promising tool for clinical isolation and analysis of CTCs.
  • The microchip enhances CTC detection efficacy and selectivity.
  • This technology has the potential to advance early cancer detection and personalized medicine.