Related Experiment Video
Updated: Jan 25, 2026

Simple and Fast Rolling Circle Amplification-Based Detection of Topoisomerase 1 Activity in Crude Biological Samples
Published on: December 2, 2022
Label-free fluorescence detection of circulating microRNAs based on duplex-specific nuclease-assisted target
Tingting Fan1, Yu Mao2, Feng Liu3
1Department of Chemistry, Tsinghua University, Beijing 100084, PR China; State Key Laboratory of Chemical Oncogenomics, Key Laboratory of Chemical Biology, the Graduate School at Shenzhen, Tsinghua University, Shenzhen 518055, PR China.
Abstract:
A simpler, more rapid and selective biosensor has been developed for the sensitive detection of circulating miRNAs (c-miRNAs) based on duplex-specific nuclease (DSN)-assisted target recycling coupled with rolling circle amplification (RCA) using SYBR Gold Dye as a signal indicator. This biosensor exhibits a tremendously low detection limit of 0.3 fM of c-miRNAs and shows high selectivity for one-base mismatched c-miRNA. In addition, most importantly, the biosensor could accurately determine the amounts of target c-miRNA in total c-miRNAs isolated from human serums and human cancer cell lines, which confirms that the biosensor could be used to quantify c-miRNAs in complex biological samples and for the clinical early diagnosis of cancers based on c-miRNAs detection.
More Related Videos
05:46Fluorescence-Based Detection of FEN1 Nuclease Activity and Screening of Small-Molecule Inhibitors
Published on: June 27, 2025
07:59Rapid and Specific Detection of Acinetobacter baumannii Infections Using a Recombinase Polymerase Amplification/Cas12a-based System
Published on: April 25, 2025
Related Concept Videos
MicroRNAs
MicroRNAs
Rolling Without Slipping
Rolling With Slipping
An object's rolling motion is characterized by its rotation around its axis, while linear motion refers to the object's translational motion along a surface. Frictional forces can...
Rolling Resistance
For instance, imagine a hard cylinder rolling on a comparatively soft surface. The cylinder's weight compresses the surface beneath it. As the cylinder moves, the material in front of it slows down due to...
Mohr's Circle for Moments of Inertia: Problem Solving
Consider a rectangular beam. The moments of inertia of the beam about the x and y axis are 2.5(107) mm4 and 7.5(107) mm4, respectively. The product of inertia is 1.5(107) mm4. Determine the principal moments of inertia and the orientation of the major and...