Related Experiment Video
Updated: Jan 25, 2026

Improvement of Bacillus subtilis Spore Enumeration and Label Analysis in Flow Cytometry
Published on: June 30, 2023
Improved Expression and Optimization of Trehalose Synthase by Regulation of Pglv in Bacillus subtilis
Hongling Liu1,2,3, Hao Liu3, Shaojie Yang1,2
1State Key Laboratory of Biobased Material and Green Papermaking (LBMP), Qilu University of Technology(Shandong Academy of Sciences), Jinan, Shandong, 250353, China.
Abstract:
Trehalose synthase (TreS) converts maltose to trehalose, which has several important functions; therefore, enhancing TreS expression is desirable. Here, a recombinant Bacillus subtilis W800N (ΔamyE)-Pglv strain was constructed to achieve enhanced expression of TreS. Process optimization strategies were developed to improve the expression level of TreS in B. subtilis W800N (ΔamyE)-Pglv. Intracellular activity of TreS was induced using 60 g/L of maltose in shake flask culture. The protein activity reached 5211 ± 134 U/g at 33 °C and pH 7.0 in Luria-Bertani medium. A fed-batch fermentation strategy was applied in a 30 L fermenter containing 18 L terrific broth to achieve high cell density by replacing glycerol with high maltose syrup as a carbon source and an inducer. After 32 h of fermentation, recombinant B. subtilis W800N (ΔamyE)-Pglv activity reached 6850 ± 287 U/g dry cell weight. Our results demonstrate the efficiency of the Pglv promoter in increasing the expression of TreS in B. subtilis W800N (ΔamyE)-Pglv.
Related Concept Videos
Regulation of Expression Occurs at Multiple Steps
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
Regulation of Expression Occurs at Multiple Steps
Regulation of Expression at Multiple Steps
Constitutive and Regulated Gene Expression
ATP Synthase: Mechanism
ATP Synthase: Structure

