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Updated: Jan 25, 2026

Fast Enzymatic Processing of Proteins for MS Detection with a Flow-through Microreactor
Published on: April 6, 2016
Instantaneous isotropic volumetric imaging of fast biological processes
Nils Wagner1, Nils Norlin1,2, Jakob Gierten3,4
1Cell Biology and Biophysics Unit, European Molecular Biology Laboratory, Heidelberg, Germany.
Abstract:
To capture highly dynamic biological processes at cellular resolution is a recurring challenge in biology. Here we show that combining selective-volume illumination with simultaneous acquisition of orthogonal light fields yields three-dimensional images with high, isotropic spatial resolution and a significant reduction of reconstruction artefacts, thereby overcoming current limitations of light-field microscopy implementations. We demonstrate medaka heart and blood flow imaging at single-cell resolution and free of motion artefacts at volume rates of up to 200 Hz.
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