Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Directing Effect of Substituents: meta-Directing Groups01:09

Directing Effect of Substituents: meta-Directing Groups

5.9K
Substituents on the benzene ring that direct an incoming electrophile to undergo substitution at the meta position are called meta directors. All meta directors either have a positive charge on the atom directly bonded to the ring or a partial positive charge. These groups function by withdrawing electrons from the ring through inductive and resonance effects. Consider the carbocation intermediates formed upon the addition of an electrophile on nitrobenzene at the...
5.9K
DNA Microarrays02:34

DNA Microarrays

20.9K
Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
20.9K
Organization of Genes02:07

Organization of Genes

73.2K
Overview
73.2K
Gene Families01:57

Gene Families

9.9K
Gene families consist of groups of genes proposed to have originated from a common ancestor. Typically these arise through events in which a gene or genes are mistakenly duplicated during cell division. Unlike their parent genes (which are subject to selection pressure to maintain function), these gene copies do not need to preserve their sequences and may evolve at a relatively faster rate.
Occasionally these regions can be adapted to take on new roles within the organism, becoming novel genes...
9.9K
Combinatorial Gene Control02:33

Combinatorial Gene Control

9.5K
Combinatorial gene control is the synergistic action of several transcriptional factors to regulate the expression of a single gene. The absence of one or more of these factors may lead to a significant difference in the level of gene expression or repression.
The expression of more than 30,000 genes is controlled by approximately 2000-3000 transcription factors. This is possible because a single transcription factor can recognize more than one regulatory sequence. The specificity in gene...
9.5K
meta-Directing Deactivators: –NO2, –CN, –CHO, –⁠CO2R, –COR, –CO2H01:13

meta-Directing Deactivators: –NO2, –CN, –CHO, –⁠CO2R, –COR, –CO2H

6.6K
All meta-directing substituents are deactivating groups. These substituents withdraw electrons from the aromatic ring, making the ring less reactive toward electrophilic substitution. For example, the nitration of nitrobenzene is 100,000 times slower than that of benzene because of the deactivating effect of the nitro group. The first step in an electrophilic aromatic substitution is the addition of an electrophile to form a resonance-stabilized carbocation. The energy diagrams for...
6.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Targeting cathepsin B by cycloastragenol enhances antitumor immunity of CD8 T cells via inhibiting MHC-I degradation.

Journal for immunotherapy of cancer·2022
Same author

The Transcription of Flight Energy Metabolism Enzymes Declined with Aging While Enzyme Activity Increased in the Long-Distance Migratory Moth, <i>Spodoptera frugiperda</i>.

Insects·2022
Same author

Effects of glucose oxidase on growth performance, clinical symptoms, serum parameters, and intestinal health in piglets challenged by enterotoxigenic <i>Escherichia coli</i>.

Frontiers in microbiology·2022
Same author

Living Cell-Target Responsive Accessibility Profiling Reveals Silibinin Targeting ACSL4 for Combating Ferroptosis.

Analytical chemistry·2022
Same author

Effects of land clearing for agriculture on soil organic carbon stocks in drylands: A meta-analysis.

Global change biology·2022
Same author

LncRNA SNHG16 promotes development of oesophageal squamous cell carcinoma by interacting with EIF4A3 and modulating RhoU mRNA stability.

Cellular & molecular biology letters·2022
Same journal

Correction.

Andrologia·2023
Same journal

The combination of ginger and zinc supplement could improve lead-induced reproductive dysfunction by inhibiting apoptosis mediated by oxidative damage and inflammation.

Andrologia·2022
Same journal

Testosterone and lifespan in males: At the right time and the right level.

Andrologia·2022
Same journal

CORRIGENDUM.

Andrologia·2022
Same journal

CORRIGENDUM.

Andrologia·2022
Same journal

Microfluidic devices employing chemo- and thermotaxis for sperm selection can improve sperm parameters and function in patients with high DNA fragmentation.

Andrologia·2022
See all related articles

Related Experiment Video

Updated: Jan 25, 2026

Global Gene Expression Analysis Using a Zebrafish Oligonucleotide Microarray Platform
13:14

Global Gene Expression Analysis Using a Zebrafish Oligonucleotide Microarray Platform

Published on: August 10, 2009

12.2K

Microarray meta-analysis identifies candidate genes for human spermatogenic arrest.

Fang Kui1,2, Hui Ye1, Xi-Ling Chen2

  • 1Clinical Laboratory, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, China.

Andrologia
|May 1, 2019
PubMed
Summary
This summary is machine-generated.

This study analyzed gene expression in human testes to understand male infertility. It identified key genes and pathways, revealing G1 mitotic cell cycle arrest as a feature of impaired spermatogenesis.

Keywords:
ABL1HDAC2cell cyclemicroarrayspermatogenesis

More Related Videos

Candidate Gene Testing in Clinical Cohort Studies with Multiplexed Genotyping and Mass Spectrometry
05:53

Candidate Gene Testing in Clinical Cohort Studies with Multiplexed Genotyping and Mass Spectrometry

Published on: June 21, 2018

10.6K
Bacterial Gene Expression Analysis Using Microarrays
29:41

Bacterial Gene Expression Analysis Using Microarrays

Published on: May 28, 2007

9.2K

Related Experiment Videos

Last Updated: Jan 25, 2026

Global Gene Expression Analysis Using a Zebrafish Oligonucleotide Microarray Platform
13:14

Global Gene Expression Analysis Using a Zebrafish Oligonucleotide Microarray Platform

Published on: August 10, 2009

12.2K
Candidate Gene Testing in Clinical Cohort Studies with Multiplexed Genotyping and Mass Spectrometry
05:53

Candidate Gene Testing in Clinical Cohort Studies with Multiplexed Genotyping and Mass Spectrometry

Published on: June 21, 2018

10.6K
Bacterial Gene Expression Analysis Using Microarrays
29:41

Bacterial Gene Expression Analysis Using Microarrays

Published on: May 28, 2007

9.2K

Area of Science:

  • Reproductive Biology
  • Genetics
  • Bioinformatics

Background:

  • Male infertility impacts many couples, with rising prevalence.
  • Human testis research is limited, necessitating advanced analytical approaches.
  • Understanding gene expression is crucial for elucidating male infertility mechanisms.

Purpose of the Study:

  • To investigate the gene expression profile of impaired human testes.
  • To identify novel hub genes and pathways associated with male infertility phenotypes.
  • To explore the role of p53-interacting proteins in spermatogenesis.

Main Methods:

  • Meta-analysis of 7 microarray datasets comprising 178 human testis samples.
  • Categorization of impaired testes into four pathological phenotypes and a normal phenotype based on Johnsen score.
  • Bioinformatics analysis of differentially expressed genes (DEGs) to identify key molecular players.

Main Results:

  • Identification of novel hub genes and pathways implicated in male infertility.
  • Discovery of G1 mitotic cell cycle arrest as a significant feature in pre-meiotic arrest.
  • Selection of fifteen p53-interacting proteins, including ABL1 and HDAC2, with potential roles in spermatogenesis.

Conclusions:

  • The study provides insights into the molecular mechanisms underlying male infertility.
  • G1 mitotic cell cycle arrest is a key event in pre-meiotic arrest during male infertility.
  • Further research into identified p53-interacting proteins may reveal new therapeutic targets for male infertility.