Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Protein-protein Interfaces02:04

Protein-protein Interfaces

14.6K
Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most PPIs are stabilized by numerous weak noncovalent chemical forces. The physical shape of the interfaces determines the way two proteins interact. Many globular proteins have closely-matching shapes on their surfaces, which form a large number of weak bonds. Additionally, many PPIs occur between two helices or between a surface cleft and a...
14.6K
Requirements for Human Life01:26

Requirements for Human Life

13.3K
The Earth and its atmosphere have provided humans with air, water, and food, but these are not the only requirements for survival. Humans also require a specific range of temperature and pressure that the Earth and its atmosphere provides.
Oxygen
Atmospheric air is only about 20 percent oxygen, but that oxygen is a key component of the chemical reactions that keep the body alive, including the reactions that produce ATP. Brain cells are susceptible to a lack of oxygen because they require a...
13.3K
Proteins: Dietary Sources and Requirements01:28

Proteins: Dietary Sources and Requirements

1.6K
Consuming animal-based products offers high-quality proteins that contain optimal levels and combinations of essential amino acids, crucial for tissue repair and growth. Foods like eggs, milk, fish, and most meats are a source of complete proteins. Legumes and cereals are abundant in proteins; however, they typically lack a full range of essential amino acids. As a result, they are considered incomplete protein sources. Some plant sources like soybeans, quinoa, and amaranth do contain complete...
1.6K
Oxygen Requirements and Growth Patterns01:29

Oxygen Requirements and Growth Patterns

1.2K
Microorganisms exhibit diverse oxygen requirements and growth patterns driven by their metabolic strategies and environmental adaptations. Oxygen, while essential for many organisms, can also be toxic under certain conditions, shaping how microorganisms grow and survive.Oxygen Requirements of MicroorganismsMicroorganisms are classified based on their ability to use or tolerate oxygen:● Obligate aerobes like Mycobacterium tuberculosis need oxygen for energy production, as it serves as the...
1.2K
Biological Effects of Radiation02:59

Biological Effects of Radiation

17.7K
All radioactive nuclides emit high-energy particles or electromagnetic waves. When this radiation encounters living cells, it can cause heating, break chemical bonds, or ionize molecules. The most serious biological damage results when these radioactive emissions fragment or ionize molecules. For example, α and β particles emitted from nuclear decay reactions possess much higher energies than ordinary chemical bond energies. When these particles strike and penetrate matter, they...
17.7K
Energy-requiring Steps of Glycolysis01:20

Energy-requiring Steps of Glycolysis

171.4K
Glucose is the source of nearly all energy used by organisms. The first step of converting glucose into usable energy is called glycolysis. Glycolysis occurs in the cytosol of the cell over two phases: an energy-requiring phase and an energy-releasing phase. Over the first three steps, glucose is converted into different forms and attached to two phosphate groups donated by two ATP molecules, resulting in an unstable sugar. In the next two stages, the unstable sugar splits into two sugar...
171.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Leveraging Pretrained Neural Network Models for the Classification of Tumor Cells Analyzed by Label-Free Phase Holotomographic Microscopy.

Computational and structural biotechnology journal·2026
Same author

Submolecular modulation of PIEZO1 mechanotransduction with wireless tailor-made nanoswitches.

Bioactive materials·2026
Same author

Biofabrication in suspension media for tissue engineering and<i>in vitro</i>modelling.

Biofabrication·2025
Same author

Label-Free Cancer Detection Methods Based on Biophysical Cell Phenotypes.

Bioengineering (Basel, Switzerland)·2025
Same author

Assessment of Lumbar Vertebrae L1-L7 and Proximal Femur Microstructure in Sheep as a Large Animal Model for Osteoporosis Research.

Biology·2025
Same author

Understanding Degeneration and Healing Pathways for Tissue-Engineered Treatment Strategies in Tendinopathy.

Cells, tissues, organs·2025

Related Experiment Video

Updated: Jan 25, 2026

Using Synthetic Biology to Engineer Living Cells That Interface with Programmable Materials
10:28

Using Synthetic Biology to Engineer Living Cells That Interface with Programmable Materials

Published on: March 9, 2017

9.6K

Enthesis Tissue Engineering: Biological Requirements Meet at the Interface.

Isabel Calejo1,2, Raquel Costa-Almeida1,2, Rui L Reis1,2,3

  • 113B's Research Group, I3Bs-Institute on Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, Guimarães, Portugal.

Tissue Engineering. Part B, Reviews
|May 1, 2019
PubMed
Summary

This review explores cell and tissue engineering for the tendon-to-bone interface. It highlights cell roles, oxygen effects, and culture systems for improved enthesis regeneration.

Keywords:
2D versus 3D culturecell-based strategiesgradient biomaterialsgrowth factorstendon-to-bone interfacetissue engineering

More Related Videos

Experimental Approaches to Tissue Engineering
16:41

Experimental Approaches to Tissue Engineering

Published on: August 30, 2007

6.8K
Micro-scale Engineering for Cell Biology
04:42

Micro-scale Engineering for Cell Biology

Published on: October 1, 2007

5.2K

Related Experiment Videos

Last Updated: Jan 25, 2026

Using Synthetic Biology to Engineer Living Cells That Interface with Programmable Materials
10:28

Using Synthetic Biology to Engineer Living Cells That Interface with Programmable Materials

Published on: March 9, 2017

9.6K
Experimental Approaches to Tissue Engineering
16:41

Experimental Approaches to Tissue Engineering

Published on: August 30, 2007

6.8K
Micro-scale Engineering for Cell Biology
04:42

Micro-scale Engineering for Cell Biology

Published on: October 1, 2007

5.2K

Area of Science:

  • Biomedical Engineering
  • Regenerative Medicine
  • Cell Biology

Background:

  • The tendon-to-bone interface (enthesis) is crucial for musculoskeletal function and healing.
  • Cellular behavior, including response to oxygen and growth factors, dictates enthesis development and regeneration.
  • Understanding these cellular requirements is key to advancing tissue engineering strategies.

Purpose of the Study:

  • To provide a comprehensive overview of cell-based and tissue-engineered strategies for the tendon-to-bone interface.
  • To review recent findings on cellular roles, oxygen concentration, and growth factor production in enthesis biology.
  • To critically evaluate 2D vs. 3D culture systems and mechanical stimulation in the context of enthesis engineering.

Main Methods:

  • Literature review of recent studies on cell-based and tissue-engineered approaches for tendon-to-bone healing.
  • Analysis of physiological factors influencing cell behavior at the enthesis, such as oxygen levels and growth factors.
  • Critical assessment of in vitro culture systems (2D vs. 3D) and mechanical stimulation techniques.

Main Results:

  • Cells play an essential role in the development, healing, and regeneration of the tendon-to-bone interface.
  • Oxygen concentration and growth factor production significantly impact the physiological status of the enthesis.
  • Both 2D and 3D culture systems, along with mechanical stimulation, offer distinct advantages and limitations for enthesis engineering.

Conclusions:

  • Advances in bioengineered strategies must align with the biological and cellular requirements of the enthesis.
  • Optimizing cell-based and tissue-engineered approaches holds significant potential for innovations in tendon-to-bone regeneration.
  • Further research integrating cellular insights into engineering strategies is needed to enhance enthesis repair and function.