Related Experiment Video
Updated: Jan 25, 2026

Use of Micropipette-Guided Drug Administration as an Alternative Method to Oral Gavage in Rodent Models
Published on: July 26, 2024
Institutional protocols for the oral administration (gavage) of chemicals and microscopic microbial communities to
Alexander Rodriguez-Palacios1,2, Mikhail V Khoretonenko3, Sanja Ilic4
11 Division of Gastroenterology and Liver Diseases, Department of Medicine, and Digestive Diseases Research Institute, Case Western Reserve University School of Medicine, Cleveland, OH 44106, USA.
Impact Statement:
Institutional protocols designed for the oral administration of live microbial communities, either complex or microscopic (microcosmic), to mice do not exist. However, this approach is increasingly employed by investigators focusing on the gut microbiome in experimental research. Herein, we propose two analytically Kappa-based consensus protocols to promote reproducibility and standardization in research practices and describe biologically relevant factors in achieving optimal microbial engraftment of communities in germ-free mice.
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