Aquiflexum aquatile sp. nov., isolated from lake water

A Gram-stain-negative, facultative anaerobic, red-coloured, rod-shaped, non-flagellated, non-motile and non-gliding bacterium, designated strain Z0201^T, was isolated from lake water in Yunnan, China (26° 16' N, 99° 94' E). Cells of strain Z0201^T were 0.2-0.4 µm wide and 1.4-2.5 µm long, catalase-positive and oxidase-negative. Strain Z0201^T was found to grow at 4-37 °C (optimum, 30 °C) and pH 6.5-8.5 (pH 7.5) in the presence of 0-2.0 % (w/v) NaCl (0-0.5 %). The sole respiratory quinone of strain Z0201^T was MK-7 and the DNA G+C content was 41.2 mol%. The major fatty acid was iso-C15 : 0 (49.4 %). The polar lipid profile of strain Z0201^T consisted of aminophospholipid, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylinositol, phosphatidylethanolamine, two unidentified phospholipids and five unidentified lipids. Based on the 16S rRNA gene sequence analysis, strain Z0201^T was a member of the genus Aquiflexum, appearing to be closely related to Aquiflexum balticum (95.4 %). On the basis of phenotypic distinctiveness and phylogenetic divergence, strain Z0201^T is considered to represent a novel species of the genus Aquiflexum, for which the name Aquiflexumaquatile sp. nov. is proposed. The type strain is Z0201^T (=KCTC 62450^T=MCCC 1H00328^T).