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Related Experiment Videos

Carbonic anhydrase activity develops postnatally in the rat optic nerve.

P K Davis, W G Carlini, B R Ransom

    Brain Research
    |February 1, 1987
    PubMed
    Summary

    Carbonic anhydrase (CA) activity emerges in rat optic nerves during development, coinciding with myelin formation. This enzyme, primarily in oligodendrocytes and myelin, influences age-dependent pH changes during neural activity.

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    Area of Science:

    • Neuroscience
    • Biochemistry
    • Developmental Biology

    Background:

    • Carbonic anhydrase (CA) is a crucial enzyme in physiological processes.
    • Its role in the central nervous system (CNS) white matter, specifically the optic nerve, is not fully understood.
    • Developmental changes in enzyme activity and their correlation with structural and functional alterations require investigation.

    Purpose of the Study:

    • To investigate the developmental appearance of carbonic anhydrase (CA) activity in rat optic nerves (RONs).
    • To correlate CA activity with oligodendrogenesis, myelinogenesis, and physiological changes in extracellular pH (pHo).
    • To determine the cellular localization of CA within the RON.

    Main Methods:

    • Assessed CA activity in RONs from postnatal day 5 to 77.

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  • Administered a mitotic inhibitor to deplete oligodendrocytes and myelin.
  • Measured extracellular pH (pHo) changes in response to neural activity in RONs of different ages.

    • Main Results:

    • CA activity was minimal before 10 postnatal days and increased thereafter.
    • CA activity development paralleled oligodendrogenesis and myelinogenesis.
    • Depletion of oligodendrocytes and myelin significantly reduced CA activity.
    • Age-dependent changes in extracellular pH responses to neural activity were observed, with larger acid shifts in older nerves.

    Conclusions:

    • Carbonic anhydrase is predominantly localized in oligodendrocytes and myelin within the rat optic nerve.
    • The developmental emergence of CA activity likely contributes to age-dependent alterations in extracellular pH regulation during neural activity in the optic nerve.