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Viral sequences required for efficient viral infection differ between two Chinese pepper mild mottle virus isolates
Man Yu1, He Liu1, Hongying Zheng2
1College of Plant Protection, Shenyang Agricultural University, Shenyang, 110866, China.
Virus Research
|May 1, 2019
Summary
Pepper mild mottle virus (PMMoV) infection in pepper plants was studied using infectious clones. Viral RNA sequences influence PMMoV
Area of Science:
- Plant Virology
- Molecular Plant Pathology
- Crop Science
Background:
- Pepper mild mottle virus (PMMoV) significantly impacts pepper yield and quality.
- Understanding PMMoV infection mechanisms is crucial for disease management.
Purpose of the Study:
- To construct and analyze infectious clones of PMMoV isolates.
- To investigate the role of specific viral RNA sequences in PMMoV infectivity and pathogenesis.
Main Methods:
- Construction of infectious clones for PMMoV Huludao (HLD) isolate.
- Generation of chimeric PMMoV mutants by sequence substitution.
- Infection assays in Nicotiana benthamiana and Capsicum annuum.
- Analysis of viral accumulation and infectivity.
Main Results:
- A coat protein (CP)-deficient PMMoV mutant showed reduced but systemic infection.
- Chimeric viruses exhibited varying infectious abilities, with some showing reduced infectivity compared to wild-types.
- Differential RNA sequence requirements for efficient PMMoV infection were identified between isolates.
Conclusions:
- Viral RNA sequences play a critical role in determining PMMoV infectivity and host range.
- Differences in RNA sequences between PMMoV isolates contribute to variations in infection efficiency.
- Findings provide insights into PMMoV pathogenesis and inform future disease control strategies.
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