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Updated: Jan 25, 2026

Enhanced Genetic Analysis of Single Human Bioparticles Recovered by Simplified Micromanipulation from Forensic ‘Touch DNA’ Evidence
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Retrieving forensic information about the donor through bacterial profiling.

Katherine Phan1, Mark Barash2, Xanthe Spindler1

  • 1Faculty of Science, School of Mathematical and Physical Sciences, Centre for Forensic Science, University of Technology Sydney, PO Box 123, Broadway, NSW, 2007, Australia.

International Journal of Legal Medicine
|May 2, 2019
PubMed
Summary
This summary is machine-generated.

Forensic science can now use bacterial profiles from fingerprints to identify donors. Certain bacteria on skin correlate with characteristics like gender, ethnicity, and diet, offering new forensic intelligence possibilities.

Keywords:
Bacterial profilingForensic microbiologyInvestigative microbiologyMassively parallel sequencing

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Area of Science:

  • Forensic microbiology
  • Human microbiome analysis

Background:

  • Fingermarks deposit skin bacteria, creating unique "bacterial profiles."
  • Individual skin microbiome diversity suggests potential for donor identification.

Purpose of the Study:

  • To investigate bacterial profiles as indicators of donor characteristics for forensic intelligence.
  • To explore correlations between skin bacteria and personal attributes.

Main Methods:

  • Forty-five participants touched playing cards, leaving fingermarks and associated bacteria.
  • Bacterial communities were analyzed using 16S rRNA sequencing.
  • Microbiome data was compared against donor characteristics.

Main Results:

  • Correlations found between bacterial profiles and gender, ethnicity, diet, and hand sanitizer use.
  • Absence of specific bacteria (Lactococcus, Alloiococcus) linked to diet and gender/ethnicity/sanitizer use.
  • Gender prediction models showed highest accuracy.

Conclusions:

  • Skin bacterial species correlate with personal characteristics of forensic relevance.
  • Microbiome genotyping presents a novel application in forensic science for donor profiling.