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High-throughput Siderophore Screening from Environmental Samples: Plant Tissues, Bulk Soils, and Rhizosphere Soils
Published on: February 9, 2019
Sampling and Handling of Soil to Identify Microorganisms with Impacts on Plant Growth
1Division of Plant Sciences, University of Missouri, Columbia, MO, USA. KremerR@missouri.edu.
Abstract:
Sampling and handling of soils and rhizosphere soil are very critical steps for obtaining representative microbial cultures and genomic material from these environments. Attention to position in the landscape of a sampling site, previous management of the site, time of year, and depth of sampling is important to assure representative samples. Detailed protocols are provided to assist environmental microbiologists and molecular biologists in proper sampling and handling of soils that serve as the source of cultures and DNA for subsequent use in important experiments carried out in the laboratory.
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