Implementing strategies to reduce singly housed male mice
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Updated: Jan 25, 2026

Handling Techniques to Reduce Stress in Mice
Published on: September 25, 2021
Garikoitz Azkona1, Juan M Caballero1
1Parc de Recerca Biomèdica de Barcelona (PRBB) Animal Facility, Spain.
Abstract:
There are different husbandry situations that lead to social isolation of male mice. Besides legal considerations, single housing has a negative impact on behavioural and molecular studies. We have implemented two strategies, the 'companion mouse' and just-weaned male grouping, to reduce the number of male mice housed singly in our facility. We have achieved a progressive reduction (42% in three years) without an increase in aggression.
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