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Generation of temperature sensitive mutations with error-prone PCR in a gene encoding a component of the spindle pole
Ngang Heok Tang1, Chii Shyang Fong2, Hirohisa Masuda3
1a Section of Neurobiology, Division of Biological Sciences, University of California , San Diego , CA , USA.
Abstract:
Temperature-sensitive (ts) mutants provide powerful tools for investigation of cellular functions of essential genes. We report here asimple procedure to generate ts mutations using error-prone PCR within pcp1 that encodes aspindle pole body (SPB) component in Schizosaccharomyces pombe. This manipulation is not restricted to pcp1, and can be suited to any essential genes involved in other processes.
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