Unaltered V̇o₂ kinetics despite greater muscle oxygenation during heavy-intensity two-legged knee extension versus...

Relative perfusion of active muscles is greater during knee extension ergometry (KE) than cycle ergometry (CE). This provides the opportunity to investigate the effects of increased O₂ delivery (Q̇o₂) on deoxygenation heterogeneity among quadriceps muscles and pulmonary oxygen uptake (V̇o₂) kinetics. Using time-resolved near-infrared spectroscopy, we hypothesized that compared with CE the superficial vastus lateralis (VL), superficial rectus femoris, and deep VL in KE would have 1) a smaller amplitude of the exercise-induced increase in deoxy[Hb + Mb] (related to the balance between V̇o₂ and Q̇o₂); 2) a greater amplitude of total[Hb + Mb] (related to the diffusive O₂ conductance); 3) a greater homogeneity of regional muscle deoxy[Hb + Mb]; and 4) no difference in pulmonary V̇o₂ kinetics. Eight participants performed square-wave KE and CE exercise from 20 W to heavy work rates. Deoxy[Hb + Mb] amplitude was less for all muscle regions in KE (P < 0.05: superficial, KE 17-24 vs. CE 19-40; deep, KE 19 vs. CE 26 μM). Furthermore, the amplitude of total[Hb + Mb] was greater for KE than CE at all muscle sites (P < 0.05: superficial, KE, 7-21 vs. CE, 1-16; deep, KE, 11 vs. CE, -3 μM). Although the amplitude and heterogeneity of deoxy[Hb + Mb] were significantly lower in KE than CE during the first minute of exercise, the pulmonary V̇o₂ kinetics was not different for KE and CE. These data show that the microvascular Q̇o₂ to V̇o₂ ratio, and thus tissue oxygenation, was greater in KE than CE. This suggests that pulmonary and muscle V̇o₂ kinetics in young healthy humans are not limited by Q̇o₂ during heavy-intensity cycling.