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Allergenicity assessment of fungal species using immunoclinical and proteomic techniques: a study on Fusarium

Debarati Dey1, Swati Gupta Bhattacharya1

  • 1Division of Plant Biology, Bose Institute (Main Campus) , Kolkata, India.

International Journal of Environmental Health Research
|May 3, 2019
PubMed

Insights

Airborne fungal spores like Fusarium lateritium cause respiratory allergies. Researchers identified a key 34 kDa allergen, Glyceraldehyde 3-phosphate dehydrogenase, from F. lateritium, offering potential for new allergy immunotherapy vaccines.

Area of Science:

  • Mycology
  • Immunology
  • Allergology

Background:

  • Airborne fungal spores are significant triggers for respiratory allergies in humans.
  • Fusarium lateritium, a common fungus, has been implicated in causing allergic reactions, particularly in India.

Purpose of the Study:

  • To investigate the allergenic potential of Fusarium lateritium.
  • To identify specific allergens responsible for F. lateritium-induced allergies.

Main Methods:

  • Proteins from F. lateritium were analyzed using 1D and 2D gel electrophoresis.
  • IgE-specific immunoblots were performed with sera from F. lateritium-sensitized patients.
  • Mass spectrometry was used to identify allergenic proteins.

Main Results:

  • Eleven F. lateritium-sensitized patients' sera revealed 8 immunoreactive bands on 1D immunoblot.
  • A prominent 34 kDa protein band was recognized by over 80% of patients, indicating it as a major allergen.
  • Mass spectrometric analysis identified this 34 kDa allergen as Glyceraldehyde 3-phosphate dehydrogenase.

Conclusions:

  • Glyceraldehyde 3-phosphate dehydrogenase is identified as a significant allergen in Fusarium lateritium.
  • This identified allergen presents a promising target for developing vaccines for immunotherapy in allergic patients.
Keywords:
2D gel electrophoresisFusarium lateritiumallergenfungal sporesmass spectrometrypopulation

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