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Related Experiment Videos

Expression of immunoglobulin lambda chains in the laboratory rat.

D Zhou, G A Leslie, K Guo

    Journal of Immunogenetics
    |August 1, 1986
    PubMed
    Summary

    Researchers developed a new monoclonal antibody (G33/11) to detect rat immunoglobulin lambda chains. This antibody aids in classifying hybridomas and understanding rat immunogenetics.

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    Area of Science:

    • Immunology
    • Genetics

    Background:

    • Rat immunoglobulin lambda chains play a role in immune responses.
    • Characterizing rat hybridomas is crucial for immunological research.

    Purpose of the Study:

    • To develop a novel monoclonal antibody for detecting rat immunoglobulin lambda chains.
    • To utilize this antibody for hybridoma classification and immunogenetic studies in rats.

    Main Methods:

    • Immunization of a BALB/c mouse with rat IgG1 myeloma IR31.
    • Fusion of spleen cells with P3.X63.Ag8.653 hybridoma parent line.
    • Isolation and characterization of the monoclonal antibody G33/11.

    Main Results:

    • A monoclonal antibody (G33/11) was successfully generated, targeting rat immunoglobulin lambda chains.
    • The antibody classified two existing rat hybridomas and identified a new lambda-bearing IgM hybridoma (G36/1).
    • Lambda-bearing immunoglobulin was detected in all tested normal inbred rat sera, ranging from 1.5% to 13% of total serum Ig.

    Conclusions:

    • The monoclonal antibody G33/11 is a valuable tool for rat immunogenetics.
    • This antibody facilitates the characterization of monoclonal antibodies produced in rats.
    • It broadens the understanding of immunoglobulin expression in rats.

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