First Community-Wide, Comparative Cross-Linking Mass Spectrometry Study
Claudio Iacobucci1, Christine Piotrowski1, Ruedi Aebersold2,3
1Department of Pharmaceutical Chemistry and Bioanalytics, Institute of Pharmacy, Charles Tanford Protein Center , Martin Luther University Halle-Wittenberg , Kurt-Mothes-Strasse 3a , 06120 Halle/Saale , Germany.
Analytical Chemistry
|May 3, 2019
Summary
Chemical cross-linking combined with mass spectrometry (XL-MS) is increasingly used for protein structure and interaction studies. This community effort establishes best practices for reproducible XL-MS experiments and data analysis.
Area of Science:
- Proteomics and Structural Biology
Background:
- Chemical cross-linking combined with mass spectrometry (XL-MS) is a growing technique for protein structure and interaction analysis.
- The increasing use of XL-MS necessitates standardized protocols for reproducible results.
Purpose of the Study:
- To summarize the current status of XL-MS techniques.
- To compare and evaluate existing cross-linking strategies.
- To establish a framework for best practice guidelines in XL-MS.
Main Methods:
- A worldwide, community-based harmonization study involving 32 participating groups.
- Comparison and evaluation of various cross-linking strategies and data analysis methods.
Main Results:
- Identified the need for consensus formats in XL-MS data analysis and reporting.
- Highlighted the importance of standardized protocols for generating accurate and reproducible XL-MS data.
Conclusions:
- This study provides a foundation for developing best practice guidelines for XL-MS.
- The ultimate goal is to assist scientists in achieving accurate and reproducible XL-MS results for protein structure and interaction studies.
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