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Updated: Jan 25, 2026

Quantification of Circular RNAs Using Digital Droplet PCR
Published on: September 16, 2022
Sensitive detection of porcine circovirus 3 by droplet digital PCR
Yuqi Liu1,2, Hecheng Meng1,2, Lei Shi1,2
1Research Institute of Food Safety and Nutrition, Jinan University, Guangzhou, Guangdong, China (Liu, Shi, Li).
Abstract:
Porcine circovirus 3 (PCV-3) is a newly emerging virus that poses a potential threat to the swine industry. We developed a sensitive assay utilizing droplet digital PCR (ddPCR) to detect PCV-3. Specificity of the assay was confirmed by the failure of amplification of DNA of other relevant viruses. The detection limit for ddPCR was 1 copy/μL, 10 times greater sensitivity than TaqMan real-time PCR (rtPCR). Both methods showed a high degree of linearity, although TaqMan rtPCR showed less sensitivity than ddPCR for clinical detection. Our findings indicate that ddPCR might offer faster and improved analytical sensitivity for PCV-3 detection.
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