Related Experiment Video

Updated: Jan 25, 2026

Profiling of Estrogen-regulated MicroRNAs in Breast Cancer Cells
16:24

Profiling of Estrogen-regulated MicroRNAs in Breast Cancer Cells

Published on: February 21, 2014

20.7K

LINC00511 knockdown enhances paclitaxel cytotoxicity in breast cancer via regulating miR-29c/CDK6 axis

Hualong Zhang1, Bin Zhao1, Xiuxia Wang2

  • 1Department of Breast and Thyroid Surgery, Shanxian Central Hospital, Heze 274300, China.

Life Sciences
|May 4, 2019
PubMed
Abstract

Insights

Long intergenic noncoding RNA 00511 (LINC00511) knockdown boosts paclitaxel efficacy in breast cancer by upregulating miR-29c and downregulating CDK6. This study reveals LINC00511 as a potential therapeutic target for overcoming drug resistance.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Drug resistance is a significant clinical challenge in breast cancer treatment.
  • Long non-coding RNAs (lncRNAs) are implicated in cancer drug resistance.
  • The role of LINC00511 in breast cancer drug resistance is currently unknown.

Purpose of the Study:

  • To investigate the effect of LINC00511 on the drug resistance of breast cancer cells.
  • To elucidate the molecular mechanism underlying LINC00511's role in breast cancer drug resistance.

Main Methods:

  • Gene expression analysis (RT-qPCR) of LINC00511, miR-29c, and CDK6 in breast cancer tissues and cells.
  • Correlation analysis between gene expressions.
  • Luciferase reporter assays, RT-qPCR, and Western blot to explore molecular interactions.
  • MTT assays and flow cytometry to assess paclitaxel cytotoxicity.

Main Results:

  • LINC00511 and CDK6 were upregulated, while miR-29c was downregulated in breast cancer.
  • LINC00511 directly suppressed miR-29c expression, and LINC00511 positively regulated CDK6.
  • Knockdown of LINC00511 enhanced paclitaxel cytotoxicity by upregulating miR-29c.
  • CDK6 was identified as a direct target of miR-29c.

Conclusions:

  • LINC00511 knockdown enhances paclitaxel cytotoxicity in breast cancer cells.
  • The mechanism involves the regulation of the miR-29c/CDK6 axis by LINC00511.
  • LINC00511 is a potential therapeutic target for overcoming paclitaxel resistance in breast cancer.
Keywords:
Breast cancerCDK6LINC00511Paclitaxel cytotoxicitymiR-29c

More Related Videos

Systems Biology of Metabolic Regulation by Estrogen Receptor Signaling in Breast Cancer10:36

Systems Biology of Metabolic Regulation by Estrogen Receptor Signaling in Breast Cancer

Published on: March 17, 2016

10.9K
Studying Triple Negative Breast Cancer Using Orthotopic Breast Cancer Model09:29

Studying Triple Negative Breast Cancer Using Orthotopic Breast Cancer Model

Published on: March 20, 2020

18.8K

Related Concept Videos

Hypothalamic-Pituitary Axis01:37

Hypothalamic-Pituitary Axis

The response to stress—be it physical or psychological, acute or chronic—involves activation of the Hypothalamic-Pituitary-Adrenal (HPA) axis. The HPA axis is part of the neuroendocrine system because it involves both neuronal and hormonal communication. Its function is to regulate homeostatic systems—metabolic, cardiovascular, and immune—providing the necessary means to respond to a stressor.
65.8K
GTPases and their Regulation02:14

GTPases and their Regulation

Guanine nucleotide-binding proteins (G-proteins), also known as GTPases, are a superfamily of proteins that regulate many cellular processes, such as cell signaling, vesicular transport, and the regulation of cell shape and motility. Mutation or dysfunction of these proteins can lead to disease. There are around 40,000 known G-proteins that can broadly be classified into two groups ‒  small G-proteins consisting of a single domain and large multi-domain G-proteins.
Large G-proteins,...
9.8K
Perpendicular-Axis Theorem01:16

Perpendicular-Axis Theorem

The perpendicular-axis theorem states that the moment of inertia of a planar object about an axis perpendicular to its plane is equal to the sum of the moments of inertia about two mutually perpendicular concurrent axes lying in the plane of the body.
Consider a circular disc of mass M and radius R lying along an x-y plane. The origin lies at the center of the disc, and the z-axis is perpendicular to the disc's plane. All three axes coincide at the disc's center. The moment of inertia of this...
4.5K
Parallel-axis Theorem01:06

Parallel-axis Theorem

The parallel-axis theorem provides a convenient and quick method of finding the moment of inertia of an object about an axis parallel to the axis passing through its center of mass. Consider a thin rod as an example. There is a striking similarity between the process of finding the moment of inertia of a thin rod about an axis through its middle, where the center of mass lies, and about an axis through its end using the conventional method. In the conventional method, the concept of linear mass...
8.2K
Master Transcription Regulators02:23

Master Transcription Regulators

Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
7.7K
Moment of Inertia about an Arbitrary Axis01:20

Moment of Inertia about an Arbitrary Axis

The moment of inertia is typically associated with principal axes, but it can also be computed for any random axis. When an arbitrary axis is under consideration, the moment of inertia is determined by integrating the mass distribution of the object along that specific axis. It is crucial in applications like the design of machinery, where components rotate about various axes, and balance and stability are essential.
In this scenario, the perpendicular distance between the chosen arbitrary axis...
621