CD39 identifies a microenvironment-specific anti-inflammatory CD8⁺ T-cell population in atherosclerotic lesions

CD8⁺ T-cells have been attributed both atherogenic and atheroprotective properties, but analysis of CD8⁺ T-cells has mostly been restricted to the circulation and secondary lymphoid organs. The atherosclerotic lesion, however, is a complex microenvironment containing a plethora of inflammatory signals, which may affect CD8⁺ T-cell activation. Here, we address how this environment affects the functionality of CD8⁺ T-cells.

We compared the cytokine production of CD8⁺ T-cells derived from spleens and enzymatically digested aortas of apoE^-/- mice with advanced atherosclerosis by flow cytometry. Aortic CD8⁺ T-cells produced decreased amounts of IFN-γ and TNF-α compared to their systemic counterparts. The observed dysfunctional phenotype of the lesion-derived CD8⁺ T-cells was not associated with classical exhaustion markers, but with increased expression of the ectonucleotidase CD39. Indeed, pharmacological inhibition of CD39 in apoE^-/- mice partly restored cytokine production by CD8⁺ T-cells. Using a bone-marrow transplantation approach, we show that TCR signaling is required to induce CD39 expression on CD8⁺ T-cells in atherosclerotic lesions. Importantly, analysis of human endarterectomy samples showed a strong microenvironment specific upregulation of CD39 on CD8⁺ T-cells in the plaques of human patients compared to matched blood samples.

Our results suggest that the continuous TCR signaling in the atherosclerotic environment in the vessel wall induces an immune regulatory CD8⁺ T-cell phenotype that is associated with decreased cytokine production through increased CD39 expression in both a murine atherosclerotic model and in atherosclerosis patients. This provides a new understanding of immune regulation by CD8⁺ T-cells in atherosclerosis.