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Updated: Jan 25, 2026

Regular Care and Maintenance of a Zebrafish Danio rerio Laboratory: An Introduction
Published on: November 18, 2012
The effects of fluorene-9-bisphenol on female zebrafish (Danio rerio) reproductive and exploratory behaviors
Ping Mi1, Qiu-Ping Zhang2, Shu-Hui Zhang1
1State Key Laboratory of Medicinal Chemical Biology, The Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Science, Nankai University, Tianjin, 300071, China.
Abstract:
Endocrine disruptor chemicals induce adverse effects to animals' development, reproduction and behavior in environment. We investigated the effects of fluorene-9-bisphenol (BHPF), one substitute of bisphenol A, on courtship behavior and exploratory behavior of adult zebrafish. Customized apparatus was used to evaluate courtship behavior. The result showed that the male spent less time with BHPF and anti-oestrogenic fulvestrant (FULV) treated female in region of approaching (ROA). Courtship index between BHPF-exposed female and male decreased. The body orientation of BHPF- and FULV-exposed female to male decreased. Furthermore, BHPF exposure downregulated the expression of genes related to estrogen receptor, steroidogenesis and upregulated oxidative stress related genes. It indicated that BHPF exposure interfered the preference of male and female in courtship, and induced detrimental effects on reproduction. BHPF treatment decreased locomotor activity and time spent in top, increased freezing bouts, and induced anxiety/depression-like behavior. The tyrosine hydroxylase in brain decreased under BHPF exposure. Here we showed the potential adverse effects of BHPF on reproduction and exploratory behaviors.
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