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Updated: Jan 25, 2026
Determination of In Vitro and Cellular Turn-on Kinetics for Fluorogenic RNA Aptamers
Published on: August 9, 2022
Ratiometric fluorogenic determination of endogenous hypochlorous acid in living cells
Jiwen Hu1, Xin Zhang2, Tingting Liu3
1School of Environmental and Chemical Engineering, Shanghai University, Shanghai, 200444, PR China; Division of Molecular Surface Physics & Nanoscience, Department of Physics, Chemistry and Biology, Linköping University, Linköping 58183, Sweden.
Abstract:
Hypochlorous acid (HClO) is one of the most important ROS (reactive oxygen species) and common pollutant in tap-water. However, the determination of HClO with fast response and high sensitivity/selectivity is still an urgent demanding. Here we fabricated a ratiometric fluorescent probe RC based on TBET (through-bond energy transfer) on the platform of coumarin and rhodamine with the thiosemicarbazide group as the linker. This probe could display the characteristic fluorescence emission of coumarin. Upon addition of HClO, the linker was reacted into an oxadiazole, resulting in the opening of spiro-ring of rhodamine. The resultant then gives ratiometric fluorogenic changes. The probe exhibits fast response and high selectivity and sensitivity towards HClO with a low limit of detection (~140 nM). Eventually, RC is successfully applicated for determining spiked HClO in water samples and imaging endogenous HClO in living cells.
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