Ampicillin Silver Nanoformulations against Multidrug resistant bacteria
Nafeesa Khatoon1, Hammad Alam1, Afreen Khan2
1Department of Biosciences, Jamia Millia Islamia, New Delhi, 110025, India.
Scientific Reports
|May 4, 2019
Summary
This study synthesized dual-action silver nanoparticles using ampicillin, showing enhanced antibacterial activity against resistant bacteria. These novel nanoparticles did not induce resistance even after repeated exposure, indicating promising biocompatibility.
Area of Science:
- Nanotechnology
- Materials Science
- Microbiology
Background:
- Antibiotic resistance is a growing global health threat.
- Development of novel antimicrobial agents is crucial.
- Silver nanoparticles (AgNPs) possess antimicrobial properties.
Purpose of the Study:
- To synthesize and characterize novel silver nanoparticles using ampicillin (Amp-AgNPs).
- To evaluate the antibacterial efficacy of Amp-AgNPs against sensitive and drug-resistant bacteria.
- To assess the potential of Amp-AgNPs to overcome bacterial resistance.
Main Methods:
- Single-step synthesis of Amp-AgNPs using ampicillin.
- Characterization using UV-VIS spectroscopy, TEM, XRD, FTIR, and TGA.
- Antibacterial activity assessed via Minimum Inhibitory Concentration (MIC) assays.
- Evaluation of Amp-AgNPs efficacy after repeated bacterial exposure.
- Biocompatibility testing using HaCaT cell lines.
Main Results:
- Amp-AgNPs were successfully synthesized and characterized.
- FTIR and TGA confirmed ampicillin's role in nanoparticle formation.
- Amp-AgNPs exhibited significantly lower MIC values (3-28 µg/ml) compared to ampicillin and chemically synthesized AgNPs.
- No bacterial resistance developed against Amp-AgNPs after 15 successive exposure cycles.
- Preliminary biocompatibility was observed with HaCaT cell lines.
Conclusions:
- Amp-AgNPs represent a novel nanoformulation with dual antibiotic and silver properties.
- Amp-AgNPs demonstrate superior antibacterial efficacy and overcome existing resistance mechanisms.
- The developed Amp-AgNPs show potential as a new therapeutic agent against bacterial infections.
- Further research into the biocompatibility and therapeutic applications of Amp-AgNPs is warranted.
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