Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Apoptosis01:30

Apoptosis

14.3K
Apoptosis is a combination of two Greek words, 'apo' and 'ptosis,' meaning separation and falling off, respectively. Hippocrates used this word to describe gangrene, which was caused due to bandaging of fractured bones. Apoptosis was distinguished from necrosis in 1970 when John Kerr reported observations of morphological changes occurring during apoptosis. During one experiment, he observed that the disruption of blood supply to the liver tissue resulted in a size...
14.3K
Acid Suppressive Drugs for Peptic Ulcer Disease: Antacids01:31

Acid Suppressive Drugs for Peptic Ulcer Disease: Antacids

654
In the complex environment of the gastric lumen, excessive acid secretion can lead to the formation or worsening of ulcers within the delicate mucosal layer. Antacids, such as sodium bicarbonate and calcium carbonate, provide relief by neutralizing this acid, transforming it into harmless salt and water. This neutralization process raises the gastric pH from a highly acidic level of 1 to a more basic 3-4, reducing the acidity within the stomach.
However, this neutralization reaction between...
654
Acid Suppressive Drugs for Peptic Ulcer Disease: Proton Pump Inhibitors01:13

Acid Suppressive Drugs for Peptic Ulcer Disease: Proton Pump Inhibitors

873
Peptic ulcers, often induced by H. pylori infections or NSAID usage, arise from disruptions in the delicate balance of gastric acid production. Peptic ulcers stem from heightened gastric acid levels due to H. pylori infections or NSAID use. The protective mucus layer diminishes in the presence of these factors, allowing gastric acid to erode the stomach lining and form ulcers.
Gastric acid, a potent cocktail of hydrogen and chloride ions, is produced in specialized parietal cells within the...
873
Acid Suppressive Drugs for Peptic Ulcer Disease: Histamine H2-Receptor Antagonists01:28

Acid Suppressive Drugs for Peptic Ulcer Disease: Histamine H2-Receptor Antagonists

966
Histamine H2 receptors, which are intricately located on the basolateral membrane of parietal cells, play a crucial role in modulating gastric acid secretion. When released from enterochromaffin-like cells, histamine engages H2 receptors, initiating the cyclic AMP (cAMP) pathway. In this pathway, adenylyl cyclase converts ATP into cAMP, elevating intracellular cAMP levels. The activation of protein kinase A follows, stimulating the proton pump. This stimulation prompts the secretion of hydrogen...
966
Responses to Gravity and Touch02:26

Responses to Gravity and Touch

41.7K
Gravitropism: Plant Responses to Gravity
41.7K
Adult Stem Cells01:33

Adult Stem Cells

33.4K
Stem cells are undifferentiated cells that divide and produce more stem cells or progenitor cells that differentiate into mature, specialized cell types. All the cells in the body are generated from stem cells in the early embryo, but small populations of stem cells are also present in many adult tissues including the bone marrow, brain, skin, and gut. These adult stem cells typically produce the various cell types found in that tissue—to replace cells that are damaged or to continuously...
33.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The working lives of neuronal 5-HT<sub>2A</sub> receptors from the outside in.

RSC chemical biology·2026
Same author

G protein-coupled receptors and inflammation resolution signaling networks in the heart: Pharmacology and potential for innovative therapeutics.

Pharmacological reviews·2026
Same author

Gαq-Stimulated Gene Expression Is Insensitive to Bromo Extra Terminal Domain Inhibitors in HEK 293 Cells.

International journal of molecular sciences·2025
Same author

Inositol Phosphate Kinase Architecture: Practical Approaches and Lessons Learned.

Methods in molecular biology (Clifton, N.J.)·2025
Same author

Gβγ Signaling: Lessons Across the Cellular Multiverse.

Annual review of pharmacology and toxicology·2025
Same author

Opioid Analgesics: Rise and Fall of Ligand Biased Signaling and Future Perspectives in the Quest for the Holy Grail.

CNS drugs·2025

Related Experiment Video

Updated: Jan 25, 2026

Real Time Detection of In Vitro Tumor Cell Apoptosis Induced by CD8+ T Cells to Study Immune Suppressive Functions of Tumor-infiltrating Myeloid Cells
09:57

Real Time Detection of In Vitro Tumor Cell Apoptosis Induced by CD8+ T Cells to Study Immune Suppressive Functions of Tumor-infiltrating Myeloid Cells

Published on: January 29, 2019

23.4K

PPIP5K1 Suppresses Etoposide-triggered Apoptosis.

Gayane Machkalyan1, Terence E Hèbert1, Gregory J Miller1,2

  • 1Department of Pharmacology and Therapeutics, McGill University, Montréal, Québec, Canada.

Journal of Molecular Signaling
|May 4, 2019
PubMed
Summary

Diphosphoinositol pentakisphosphate kinase 1 (PPIP5K1) reduces cell sensitivity to genotoxic agents by decreasing p53 phosphorylation. This contrasts with inositol hexakisphosphate kinase 2 (IP6K2), highlighting distinct roles in apoptosis.

Keywords:
apoptosisapoptosis arrayetoposideinositol kinaseinositol pyrophosphatesp53

More Related Videos

Activation of Apoptosis by Cytoplasmic Microinjection of Cytochrome c
07:42

Activation of Apoptosis by Cytoplasmic Microinjection of Cytochrome c

Published on: June 29, 2011

17.8K
Strategies for Tracking Anastasis, A Cell Survival Phenomenon that Reverses Apoptosis
12:55

Strategies for Tracking Anastasis, A Cell Survival Phenomenon that Reverses Apoptosis

Published on: February 16, 2015

18.6K

Related Experiment Videos

Last Updated: Jan 25, 2026

Real Time Detection of In Vitro Tumor Cell Apoptosis Induced by CD8+ T Cells to Study Immune Suppressive Functions of Tumor-infiltrating Myeloid Cells
09:57

Real Time Detection of In Vitro Tumor Cell Apoptosis Induced by CD8+ T Cells to Study Immune Suppressive Functions of Tumor-infiltrating Myeloid Cells

Published on: January 29, 2019

23.4K
Activation of Apoptosis by Cytoplasmic Microinjection of Cytochrome c
07:42

Activation of Apoptosis by Cytoplasmic Microinjection of Cytochrome c

Published on: June 29, 2011

17.8K
Strategies for Tracking Anastasis, A Cell Survival Phenomenon that Reverses Apoptosis
12:55

Strategies for Tracking Anastasis, A Cell Survival Phenomenon that Reverses Apoptosis

Published on: February 16, 2015

18.6K

Area of Science:

  • Cell Biology
  • Biochemistry
  • Molecular Biology

Background:

  • Inositol hexakisphosphate kinase 2 (IP6K2) enhances apoptosis and sensitizes cells to cytotoxic agents.
  • Diphosphoinositol pentakisphosphate kinase (PPIP5K) produces distinct inositol pyrophosphates (InsPPs) with unclear roles in cell viability.

Purpose of the Study:

  • To investigate the impact of PPIP5K1 on cellular sensitivity to genotoxic agents.
  • To determine if PPIP5K1 and IP6K2 have similar roles in regulating apoptosis.

Main Methods:

  • Overexpression of PPIP5K1 in mammalian cells.
  • Testing cellular sensitivity to cytotoxic agents (etoposide, cisplatin, sulindac).
  • Analysis of apoptosis markers, including p53 phosphorylation.

Main Results:

  • PPIP5K1 overexpression decreased sensitivity to etoposide, cisplatin, and sulindac.
  • PPIP5K1 reduced p53 phosphorylation at Ser-15, -46, and -392.
  • A kinase-impaired PPIP5K1 mutant did not protect cells, indicating catalytic activity is essential.

Conclusions:

  • PPIP5K1 protects cells from genotoxic agents, contrasting with IP6K2's pro-apoptotic role.
  • PPIP5K1's protective effect is dependent on its catalytic activity.
  • These findings reveal distinct functions of PPIP5K1 and IP6K2 in controlling cell death.