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Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
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Related Experiment Video

Updated: Jan 25, 2026

Biomolecular Imaging of Cellular Uptake of Nanoparticles using Multimodal Nonlinear Optical Microscopy
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Molecular Imaging Cellular SPIO Uptake with Nonlinear Optical Microscopy.

Lina Machtoub1, Rudolf Pfeiffer2, Aleksandar Backovic3

  • 1Universitaetsklinik Fuer Radiodiagnostik, Innsbruck Medical University, Innsbruck, Austria.

Journal of Medical Imaging and Radiation Sciences
|May 5, 2019
PubMed
Summary
This summary is machine-generated.

Researchers visualized lipid binding to super paramagnetic iron oxide (SPIO) nanoparticles in reticuloendothelial system cells using SECARS microscopy. This novel application reveals selective lipid association with SPIO in Kupffer cells and spleen melanomacrophages.

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Area of Science:

  • Biomedical imaging
  • Nanotechnology
  • Cell biology

Background:

  • Super paramagnetic iron oxide (SPIO) nanoparticles are used in biomedical imaging and drug delivery.
  • Understanding nanoparticle-cell interactions is crucial for optimizing their therapeutic and diagnostic applications.
  • The reticuloendothelial system (RES) is a key site for SPIO nanoparticle uptake.

Purpose of the Study:

  • To develop and demonstrate a novel method for visualizing the selective binding of lipids to SPIO nanoparticles within RES cells.
  • To investigate the specific interactions between SPIO nanoparticles and lipid molecules in Kupffer cells and spleen melanomacrophages.

Main Methods:

  • Utilized surface-enhanced coherent anti-Stokes Raman scattering (SECARS) microscopy for high-resolution vibrational microspectroscopy.
  • Experimentally injected SPIO nanoparticles intravenously into New Zealand White rabbits.
  • Compared SPIO-injected rabbits with control groups to identify nanoparticle-specific cellular changes.

Main Results:

  • Observed significant cellular intensity enhancements in the spectral range of 2850-2875 cm⁻¹ in hepatic Kupffer cells and spleen melanomacrophages of SPIO-injected animals.
  • These spectral enhancements were absent in control animals, indicating SPIO-specific molecular changes.
  • The observed enhancements correlate with the selective association of lipid molecules to the uptaken SPIO nanoparticles.

Conclusions:

  • SECARS microscopy provides a unique capability to visualize the selective binding of lipids to SPIO nanoparticles in RES cells.
  • This finding advances the understanding of nanoparticle-lipid interactions within the cellular environment.
  • The developed application holds potential for enhanced diagnostics and targeted therapies involving SPIO nanoparticles.