Related Experiment Video

Updated: Jan 25, 2026

Establishment of Epstein-Barr Virus Growth-transformed Lymphoblastoid Cell Lines
06:38

Establishment of Epstein-Barr Virus Growth-transformed Lymphoblastoid Cell Lines

Published on: November 8, 2011

41.2K

Efficient Epstein-Barr Virus Progeny Production Mediated by Cancer-Derived LMP1 and Virally-Encoded microRNAs

Misako Yajima1, Mamiko Miyata2, Kazufumi Ikuta3

  • 1Division of Microbiology, Faculty of Medicine, Tohoku Medical and Pharmaceutical University, Sendai 983-8536, Japan. yajima@tohoku-mpu.ac.jp.

Microorganisms
|May 5, 2019
PubMed

Insights

Cancer-derived Epstein-Barr virus (EBV) latent membrane protein 1 (LMP1) and viral microRNAs (miRNAs) enhance progeny virus production. This synergy contributes to EBV-driven epithelial cancers.

Area of Science:

  • Virology
  • Molecular Biology
  • Cancer Research

Background:

  • Epstein-Barr virus (EBV) genomes exhibit strain heterogeneity, particularly in latent genes.
  • The functional differences of EBV-encoded latent membrane protein 1 (LMP1) between cancer-associated and non-cancerous strains remain unclear.

Purpose of the Study:

  • To investigate the functional differences of cancer-derived LMP1 within the context of the EBV genome.
  • To elucidate the roles of LMP1 and viral microRNAs (miRNAs) in EBV replication and epithelial carcinogenesis.

Main Methods:

  • Utilized bacterial artificial chromosome (BAC)-cloned EBV genomes containing all viral miRNA genes.
  • Stably transfected HEK293 cells with EBV-BAC clones encoding either nasopharyngeal carcinoma (NPC)-derived CAO-LMP1 (LMP1CAO) or prototype B95-8 strain LMP1 (LMP1B95-8).
  • Assessed the generation of stable transformants and progeny virus production, including experiments with deleted viral miRNA genes.

Main Results:

  • EBV-BAC DNA encoding LMP1CAO generated significantly more stable transformants than LMP1B95-8 in HEK293 cells.
  • Stably transfected cells showed highly efficient production of progeny virus.
  • Deletion of viral miRNA genes impaired progeny virus production, indicating a synergistic role with LMP1.

Conclusions:

  • Cancer-derived LMP1 and viral miRNAs are crucial for efficient EBV progeny virus production.
  • This enhanced viral production contributes to EBV-mediated epithelial carcinogenesis.
  • Strain-specific variations in LMP1 and miRNAs play a significant role in EBV pathogenesis.
Keywords:
BARTEpstein-Barr virusLMP1Nasopharyngeal carcinomamicroRNA

More Related Videos

Isolation and Quantification of Epstein-Barr Virus from the P3HR1 Cell Line09:14

Isolation and Quantification of Epstein-Barr Virus from the P3HR1 Cell Line

Published on: September 28, 2022

4.6K
An Efficient and Simple Method to Establish NK and T Cell Lines from Patients with Chronic Active Epstein-Barr Virus Infection09:43

An Efficient and Simple Method to Establish NK and T Cell Lines from Patients with Chronic Active Epstein-Barr Virus Infection

Published on: March 30, 2018

9.7K

Related Concept Videos

MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
24.1K
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.9K
What are Viruses?00:50

What are Viruses?

Overview
127.9K
Production Efficiency01:01

Production Efficiency

Net production efficiency (NPE) is the efficiency at which organisms assimilate energy into biomass for the next trophic level. Due to low metabolic rates and less energy spent on thermoregulatory processes, the NPE of ectotherms (cold-blooded animals) is 10 times higher than endotherms (warm-blooded animals).
18.2K
Viral Recombination00:57

Viral Recombination

Cells are sometimes infected by more than one virus at once. When two viruses disassemble to expose their genomes for replication in the same cell, similar regions of their genomes can pair together and exchange sequences in a process called recombination. Alternatively, viruses with segmented genomes can swap segments in a process called reassortment.
25.0K
Viral Structure00:56

Viral Structure

Viruses are extraordinarily diverse in shape and size, but they all have several structural features in common. All viruses have a core that contains a DNA- or RNA-based genome. The core is surrounded by a protective coat of proteins called the capsid. The capsid is composed of subunits called capsomeres. The capsid and genome-containing core are together known as the nucleocapsid.
74.1K