Ultra-sensitive biomolecular detection by external referencing optofluidic microbubble resonators.

Area of Science:

• Optofluidics
• Biomolecular Detection
• Resonator Systems

Background:

• Optofluidic microbubble resonator systems offer potential for sensitive detection.
• Existing systems may face challenges with stability and noise.

Purpose of the Study:

• To propose and validate an external referencing optofluidic microbubble resonator system (EROMBRS).
• To demonstrate EROMBRS for both nonspecific and specific biomolecular detection.
• To achieve ultra-low detection limits.

Main Methods:

• Utilized an external referencing optofluidic microbubble resonator system (EROMBRS).
• Performed nonspecific detection using bovine serum albumin (BSA) molecules.
• Conducted specific detection using D-biotin molecules.

Main Results:

• Achieved good long-term stability and low noise characteristics with the EROMBRS.
• Demonstrated effective nonspecific detection of bovine serum albumin (BSA).
• Achieved ultra-low practical detection limits of 1 fg/mL for both BSA and D-biotin.

Conclusions:

• The proposed EROMBRS is an effective platform for highly sensitive biomolecular detection.
• EROMBRS demonstrates significant potential for various applications requiring ultra-low detection limits.
• The system's stability and low noise are crucial for achieving high sensitivity.