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Related Experiment Video

Updated: Jan 25, 2026

Evaluation and Manipulation of Neural Activity Using Two-Photon Holographic Microscopy

Evaluation and Manipulation of Neural Activity Using Two-Photon Holographic Microscopy

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Photonic emitter manipulation to sample nanoscale topography.

N Mateos, R Molenaar, M M A E Claessens

|May 5, 2019

Summary

This study shows how to image nanoscale surface topography using photonic emitter manipulation. A metallic probe alters fluorescence lifetime, enabling precise measurement of sample height variations in confocal imaging.

Area of Science:

Optics and Photonics
Nanotechnology
Surface Science

Background:

Confocal imaging typically provides limited resolution for nanoscale topography.
Fluorescence lifetime is sensitive to the local photonic environment of emitters.

Purpose of the Study:

To develop a novel method for imaging nanoscale surface topography using photonic emitter manipulation.
To leverage the distance-dependent interaction between a metallic probe and fluorophores.

Main Methods:

Raster-scanning a metallic probe over a fluorescently labeled sample.
Analyzing fluorescence decay at each probe position to determine probe-sample distance.
Utilizing confocal imaging to record fluorescence lifetime changes.

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Evaluation and Manipulation of Neural Activity Using Two-Photon Holographic Microscopy

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Published on: September 27, 2011

Main Results:

Demonstrated imaging of nanoscale topography with 14 nm resolution.
Showcased the ability to resolve microfabricated steps.
Confirmed the distance-dependent manipulation of fluorophore photonic environments by a metallic probe.

Conclusions:

Photonic emitter manipulation offers a viable approach for high-resolution surface topography imaging.
This technique enhances the capabilities of confocal microscopy for nanoscale surface characterization.
The method is sensitive to probe-sample proximity, enabling topographic mapping.